Example sentences for: morpholino

How can you use “morpholino” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • MgSO 4 , (ethylene-bis [oxyethylenenitrilo])tetraacetic acid (EGTA), GTP (type II-S), glycerol, 2-( N -morpholino)-ethane sulfonic acid (MES), Nonidet P 40, piperazine- N,N '-bis(2-ethanesulfonic acid) (PIPES) and Triton X-100 were purchased from Sigma Chemical Company.

  • BCIP = 5-bromo-4-chloroindol-3-yl phosphate; cDNA = complementary DNA; EGTA = (ethylene-bis [oxyethylenenitrilo])tetraacetic acid; CHTN = Cooperative Human Tissue Network; ELISA = enzyme-linked immunosorbent assay; MAP = microtubule associated protein; MES = 2-( N -morpholino)-ethane sulfonic acid; NBT = nitro blue tetrazolium; PBS = phosphate-buffered saline; PC-tubulin = MAP-free phosphocellulose-purified tubulin; PCR = polymerase chain reaction; PIPES = piperazine- N,N '-bis(2-ethanesulfonic acid); PVDF = poly(vinylidene difluoride).

  • RNA was electrophoresed in a 1% agarose gel (Molecular Biology Certified Agarose, Biorad, Hercules, CA) containing 0.66 M formaldehyde in 1× MOPS (3-(N-morpholino)-propanesulfonic acid) buffer.

  • The sequences for the oligonucleotides were the same as previously described [ 50 ] except that we used phosphothioated oligos rather than morpholino oligos.

  • 24 cells as described previously [ 44 ] . To prepare lipid suspension, phosphatidylserine and phosphatidylinositol (1:1) were sonicated in 25 mM 3-(N-morpholino)propanesulfonic acid (MOPS; pH 7.0), 1 mM EGTA, 1 mM Na 3 VO 4 . Cell extracts were assayed in 25 mM MOPS (pH 7.5), 5 mM MgCl 2 , 1 mM EGTA, 1 mM Na 3 VO 4 , 130 μM ATP, 20 μCi [ 32P]-ATP (3000 Ci/mmol) and 0.2 mg/ml sonicated lipids.


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