Example sentences for: monolayers

How can you use “monolayers” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Next, cell monolayers were lysed by scraping into 1.0 mL of lysis buffer (0.

  • mRNA was isolated from monolayers of infected cells by direct lysis in FastTrack 2.0 binding buffer (Invitrogen), and subsequent oligo(dT) purification as per the manufacturer's instructions.

  • At the end of the incubation period, the supernatant was removed and the monolayers were washed twice with HBSS.

  • For the infectivity analysis of biotinylated Ad5.CMV-LacZ, dilutions of biotinylated viral vectors were placed over monolayers of D-17 target cells on a 96-well cell culture plate (8 × 10 3cells per well), followed by the incubation of cells at 37°C for 48 h.

  • Cell monolayers were washed with PBS three times and then lysed in lysis buffer (50 mM Tris-HCl, pH 7.5, 150 mM KCl, 1 mM dithiothreitol, 1 mM EDTA, 10 μg/ml leupeptin, 2 μg/ml Aprotinin, and 0.1 mM PMSF) by subjecting the cells to 3 cycles of freezing in liquid nitrogen and thawing in a 37°C water bath.


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast