Words similar to monolayers

Example sentences for: monolayers

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The TK-1 lymphocyte cell line used in this assay expresses high levels of the α4β7 integrin, [ 39 41 ] which can interact with multiple ligands including mucosal addressin-1 (MAdCAM-1), as well as VCAM-1, L-selectin and fibronectin [ 42 ] . In this system, TNF-α stimulated TK-1 adhesion to SVEC4-10 endothelial cells is at least 50% MAdCAM-1 dependent [ 41 ] . SVEC monolayers were grown in 48-well plates as described, and to activate endothelium, the monolayers were incubated with TNF-α (1 ng/ml) for 24 h.
Having established that IL-10 gene transfection exerts a significant protective effect against TNF-α stimulated endothelial MAdCAM-1 induction, we next examined the effects of IL-10 gene transfection on the adhesion of α4β7 expressing lymphocytes (using the mouse cell line TK-1) to endothelial monolayers.
DETA-NONOate did not affect lymphocyte adhesion to untreated endothelial monolayers ( data not shown ).
Monolayers were wounded using a 1 ml plastic micropipette tip and incubated at 37°C in DMEM containing 10% FBS or in DMEM containing 10% FBS and SPARC protein purified from human platelets (48 μg/ml; Calbiochem, La Jolla, CA).
To this end, we treated primary cultures of GC and monolayers of the immortalized granulosa cell line PGC-2 [ 19 ] with the K +channel antagonist 4-aminopyridine (4-AP).