Example sentences for: monolayers

How can you use “monolayers” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Concentrated bacteria were diluted into DME + 10% FBS and distributed to antibiotic-free Caco-2 monolayers.

  • Confluent monolayers of CV-1 cells were infected with 5 × 10 5pfu/well (2.

  • Supernatants obtained from PBMC exposed to IL-2 or OPTI-MEM (control) for 3 and 24 h were overlayed on confluent monolayers of HUVEC in T175 flasks (Falcon) at 37°C in 5% CO 2 for 3 h.

  • Whole cell lysates were made from GC monolayers by standard techniques using a lysis buffer consisting of PBS with 1% Nonidet P40, 0.5% sodium deoxycholate, 0.1% SDS and protease inhibitor cocktail (1:100, Sigma P8340).

  • Monolayers were wounded using a 1 ml plastic micropipette tip and incubated at 37°C in DMEM containing 10% FBS or in DMEM containing 10% FBS and SPARC protein purified from human platelets (48 μg/ml; Calbiochem, La Jolla, CA).


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