Example sentences for: monolayer

How can you use “monolayer” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The endothelial monolayer in vivo acts as a signal transduction interface for mechanical forces, in particular, shear stress.

  • The impact of ECM (collagen) on α-SMA accumulation was also evaluated in two other experimental settings (data not shown): monolayer cultures of fibroblasts grown with ascorbic acid to permit collagen secretion, and monolayer cultures of fibroblasts grown on collagen I coated plates.

  • For example, mouse embryonal stem (ES) cells with homozygous deletion of the PTEN gene exhibit increased anchorage-independent growth as compared to normal ES cells [ 12 ] . Similarly, transfer of a wild type PTEN gene into anchorage-independent human glioblastoma cells (which lack functional PTEN), results in their greatly reduced ability to form colonies in soft agar [ 4 5 6 ] . The interpretation of these latter findings, however, is complicated by the strong anti-proliferative effects of PTEN even in monolayer culture, which is consistently observed when the wild type version of this gene is introduced into PTEN-negative tumor cells [ 4 6 7 8 9 10 18 33 ] . Moreover, in human glioma and breast cancer cell lines, the ectopic expression of wild type PTEN leads to anoikis, which is apoptosis initiated by the disruption of cell matrix-interactions [ 23 34 35 36 ] .

  • After incubation, the content of the luminal chamber was cleared and the HUVEC monolayer was washed once with 1× Dulbecco PBS (DPBS) (Biofluids).

  • We found that BRCA1 antisense cells, both as pooled populations and individual subclones, also exhibited enhanced growth in monolayer culture on plastic in the presence of estrogen compared with control vector-infected colonies.


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