Words similar to monolayer
Example sentences for: monolayer
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In comparison, repair of the wounded SPARC-null fibroblast monolayer was retarded (Fig.
The lysosomal population was first labeled with a Cascade Blue-conjugated dextran as previously described [ 3 ] . The cell monolayer was then incubated with 200 μg/mL OxyBURST Green H 2 HFF BSA in complete culture medium at 37°C for 2 hours.
Rabbit polyclonal antibody against RSV-P protein has been described [ 21 ] . Indirect immunofluorescence and nuclear staining with DAPI was performed essentially as described previously [ 33 ] . A549 cells in monolayer, grown on cover slips, were washed in PBS and fixed in ice-cold 10% trichloracetic acid for 15 min, followed by successive washes in cold 70%, 90% and absolute ethanol for 3 min each.
The wound in a monolayer of SPARC-null fibroblasts, whether cultured in the presence of mitomycin C or in its absence, remained unrepaired at 15 hours, began to show slight closure at 24 hours and healed by 48 hours (Fig.
For example, mouse embryonal stem (ES) cells with homozygous deletion of the PTEN gene exhibit increased anchorage-independent growth as compared to normal ES cells [ 12 ] . Similarly, transfer of a wild type PTEN gene into anchorage-independent human glioblastoma cells (which lack functional PTEN), results in their greatly reduced ability to form colonies in soft agar [ 4 5 6 ] . The interpretation of these latter findings, however, is complicated by the strong anti-proliferative effects of PTEN even in monolayer culture, which is consistently observed when the wild type version of this gene is introduced into PTEN-negative tumor cells [ 4 6 7 8 9 10 18 33 ] . Moreover, in human glioma and breast cancer cell lines, the ectopic expression of wild type PTEN leads to anoikis, which is apoptosis initiated by the disruption of cell matrix-interactions [ 23 34 35 36 ] .
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