Example sentences for: monolayer

How can you use “monolayer” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Progression of granulation tissue to neodermis requires a decrease in cellularity through apoptosis of endothelial cells, fibroblasts and myofibroblasts [ 28 29 ] . Keloid fibroblasts demonstrate aberrant apoptotic behavior [ 30 31 ] although studies have given variable results [ 5 9 11 30 31 32 33 34 35 ] . Our initial data for serum-starvation-induced apoptosis in monolayer cultures of dermal fibroblasts demonstrated delayed apoptosis of keloid fibroblasts and a negative correlation with α-SMA expression [ 36 ] . Similar correlations were observed in rat lung fibroblasts, where TGFβ1 increased α-SMA content while acting as an antiapoptotic agent [ 37 38 ] . It should be noted however, that many of the myofibroblasts were still able to undergo apoptosis consistent with in vivo data on palatal wound healing [ 39 ] . Thus, TGFβ1 can promote both α-SMA expression in the initial stages of wound healing and apoptosis in later stages of wound healing.

  • Centrifuged pellets of unattached cells were combined with the appropriate monolayer lysate sample and the samples were heated to 100°C for 5 min.

  • Two milliliters of EBM-2 medium + 1% human plasma were then added to the monolayer and the cells were incubated at 37°C in 5% CO 2 for 1 h.

  • The insert containing the HUVEC monolayer was then discarded, and the optical density (OD) of the fluid in the abluminal chamber was measured at 620 nm using DPBS alone as blank control.

  • In our study, LPS and lipid A samples from P. aeruginosa 9027 and B. cepacia 25416 were immobilized on a hydrophobic HPA sensor chip (BR-1000-30, Biacore AB) that was found, in our preliminary experiments, to provide an optimal matrix for LPS and lipid A. The surface of HPA sensor chips is composed of long-chain alkanethiol molecules that form a flat, quasi-crystalline hydrophobic layer that facilitates hydrophobic adsorption of liposomes or micelles on a user-defined polar lipid monolayer.


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