Example sentences for: microtubule

How can you use “microtubule” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To ensure that arginine-Sepharose chromatography did not preferentially fractionate specific tubulin isotypes, microtubule protein fractions from an early stage in purification (tubulin plus MAPs) were examined by ELISAs and Western blotting.

  • BCIP = 5-bromo-4-chloroindol-3-yl phosphate; cDNA = complementary DNA; EGTA = (ethylene-bis [oxyethylenenitrilo])tetraacetic acid; CHTN = Cooperative Human Tissue Network; ELISA = enzyme-linked immunosorbent assay; MAP = microtubule associated protein; MES = 2-( N -morpholino)-ethane sulfonic acid; NBT = nitro blue tetrazolium; PBS = phosphate-buffered saline; PC-tubulin = MAP-free phosphocellulose-purified tubulin; PCR = polymerase chain reaction; PIPES = piperazine- N,N '-bis(2-ethanesulfonic acid); PVDF = poly(vinylidene difluoride).

  • During this transition, tightly-regulated post-translational phosphorylation-dephosphorylation events and proteasome-mediated proteolytic reactions regulate the activation and inactivation of signal transduction pathways that control chromatin condensation, nuclear membrane dissolution, microtubule nucleation, and formation of a haploid oocyte [ 8 9 10 ] . Several kinases that exert major roles during OM include maturation promoting factor (MPF) [ 9 11 ] , mitogen-activated protein kinases (MAPKs) [ 12 13 ] , and the product of the c-mos protooncogene Mos kinase [ 14 15 ] . Also, during OM oocytes undergo two highly-regulated metaphase-anaphase transitions (MAT) in which homologous chromosomes are equally and randomly segregated to an oocyte and first polar body and a subsequent division in which equational division of sister chromatids results in a haploid oocyte and a second polar body.

  • There are reports of tubulin isotypes that are differentially incorporated into microtubule structures [ 62 ] . However, it is not possible to fractionate microtubules from total cytoplasmic tubulin with quick-frozen human tissue samples.

  • Myosin, the actin motor protein, has an extended coiled-coil domain necessary for the assembly of the muscle thick filaments [ 7 ] . Kinesin and dynein, two microtubule motor proteins, also contain coiled-coil domains [ 8 9 ] .


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