Words similar to microtiter
Example sentences for: microtiter
How can you use “microtiter” in a sentence? Here are some example sentences to help you improve your vocabulary:
For cytokines assays, 1 × 10 6transduced cells were incubated on anti-CD3 coated plates for 72 h at 37°C/5% CO 2 . Culture supernatants were then harvested and an ELISA assay was performed to measure the production of IL-4, IL-10 and IFN-γ as previously described [ 30 ] . To assay proliferation, the transduced cells were plated in duplicate in microtiter plates (5 × 10 5/well) at a ratio of 1:5 with irradiated BALB/c splenocytes and the indicated doses of OVA peptide at 37°C/5% CO 2 . After 72 h, 50 μCi/ml [ 3H]thymidine (Amersham, Piscataway, NJ) was added to the well.
First round amplifications were performed in 96-well microtiter dish format using PTC-100 Thermocyclers from MJ Research Inc.
Cell lysis was monitored by fluorescent microscopy and aliquots of column fractions were assayed in the wells of 96 well microtiter plates for green fluorescence using a Molecular Dynamics Storm Phosphorlmager.
HeLa cells were grown in DMEM (Gibco/BRL) supplemented with 10% fetal calf serum, seeded at 10 cells/well in 96-well microtiter plates and transfected 8 hours later using Exgen 500 (Euromedex, Souffelweyersheim, France).
About 500-1000 colonies were picked and grown in 96-well microtiter plates in LB medium with 100 mg/1 ampicillin.