Example sentences for: microtiter

How can you use “microtiter” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For cytokines assays, 1 × 10 6transduced cells were incubated on anti-CD3 coated plates for 72 h at 37°C/5% CO 2 . Culture supernatants were then harvested and an ELISA assay was performed to measure the production of IL-4, IL-10 and IFN-γ as previously described [ 30 ] . To assay proliferation, the transduced cells were plated in duplicate in microtiter plates (5 × 10 5/well) at a ratio of 1:5 with irradiated BALB/c splenocytes and the indicated doses of OVA peptide at 37°C/5% CO 2 . After 72 h, 50 μCi/ml [ 3H]thymidine (Amersham, Piscataway, NJ) was added to the well.

  • First round amplifications were performed in 96-well microtiter dish format using PTC-100 Thermocyclers from MJ Research Inc.

  • Cell lysis was monitored by fluorescent microscopy and aliquots of column fractions were assayed in the wells of 96 well microtiter plates for green fluorescence using a Molecular Dynamics Storm Phosphorlmager.

  • HeLa cells were grown in DMEM (Gibco/BRL) supplemented with 10% fetal calf serum, seeded at 10 cells/well in 96-well microtiter plates and transfected 8 hours later using Exgen 500 (Euromedex, Souffelweyersheim, France).

  • About 500-1000 colonies were picked and grown in 96-well microtiter plates in LB medium with 100 mg/1 ampicillin.


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