Words similar to microgram

Example sentences for: microgram

How can you use “microgram” in a sentence? Here are some example sentences to help you improve your vocabulary:

One microgram of total RNA was made into a cDNA library by a reverse transcription process using the following conditions: 0.1 ug of oligothymidine, 100 U of reverse transcriptase, 5 ug bovine serum albumin (BSA), 10 U RNAsin (Promega, Madison WI) in a final volume of 10 ul buffer containing 50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl 2 , 10 mM dithiothreitol, 0.5 mM each of CTP, ATP, GTP, TTP, incubated for 2 h at 37°C, and denatured at 95°C for 10 minutes.
0% Bacto-tryptone, 0.5% Yeast extract and 1.0% NaCl) with antibiotics at the following concentrations when necessary: ampicillin (Amp) at 50 microgram/ml together with methicillin at 200 microgram/ml, chloramphenicol (Cml) at 25 microgram/ml, kanamycin (Kan) at 10 microgram/ml and tetracycline (Tet) at 10 microgram/ml.
For RT reactions, one microgram of total RNAs was reverse transcribed from random (dN)6 primers in 20 μl of reaction medium containing 1 mM each deoxynucleoside triphosphate, (dN)6 (2 mM), 20 U of RNAsin, 50 mM Tris-HCl, 75 mM KCl, 5 mM MgCl 2 , and 50 U of Moloney murine leukemia virus reverse transcriptase (Applied Biosystem).
E. coli cells were grown in M9 medium (1 × M9 salts [ 34 ] , 0.2% glucose, 0.05 mM CaCl 2 , 0.5 mM MgSO 4 , 0.2% casamino acids and 1 microgram/ml vitamin B1) and LB broth (1.
This contrasts with typical mRNA expression analyses where microgram quantities of mRNA are available for direct analysis.