Words similar to microgram
Example sentences for: microgram
How can you use “microgram” in a sentence? Here are some example sentences to help you improve your vocabulary:
One microgram each of normal and heat-shocked testicular poly(A) RNA was submitted to Genome Systems (now part of IGI) for contract hybridization against their mouse Gene Expression Microarray I (GEM 1 [ 20]).
One microgram of RNA was also hybridized against 5,184-element human GeneFilter membrane arrays (RGI; GeneFilters I to IV, and Named-Genes) following purification of the [ 33P]dCTP-labeled probe with BioSpin6 columns (BioRad).
This contrasts with typical mRNA expression analyses where microgram quantities of mRNA are available for direct analysis.
One microgram of total RNA was made into a cDNA library by a reverse transcription process using the following conditions: 0.1 ug of oligothymidine, 100 U of reverse transcriptase, 5 ug bovine serum albumin (BSA), 10 U RNAsin (Promega, Madison WI) in a final volume of 10 ul buffer containing 50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl 2 , 10 mM dithiothreitol, 0.5 mM each of CTP, ATP, GTP, TTP, incubated for 2 h at 37°C, and denatured at 95°C for 10 minutes.
0% Bacto-tryptone, 0.5% Yeast extract and 1.0% NaCl) with antibiotics at the following concentrations when necessary: ampicillin (Amp) at 50 microgram/ml together with methicillin at 200 microgram/ml, chloramphenicol (Cml) at 25 microgram/ml, kanamycin (Kan) at 10 microgram/ml and tetracycline (Tet) at 10 microgram/ml.