Example sentences for: microgram

How can you use “microgram” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • One microgram each of normal and heat-shocked testicular poly(A) RNA was submitted to Genome Systems (now part of IGI) for contract hybridization against their mouse Gene Expression Microarray I (GEM 1 [ 20]).

  • One microgram of RNA was also hybridized against 5,184-element human GeneFilter membrane arrays (RGI; GeneFilters I to IV, and Named-Genes) following purification of the [ 33P]dCTP-labeled probe with BioSpin6 columns (BioRad).

  • This contrasts with typical mRNA expression analyses where microgram quantities of mRNA are available for direct analysis.

  • One microgram of total RNA was made into a cDNA library by a reverse transcription process using the following conditions: 0.1 ug of oligothymidine, 100 U of reverse transcriptase, 5 ug bovine serum albumin (BSA), 10 U RNAsin (Promega, Madison WI) in a final volume of 10 ul buffer containing 50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl 2 , 10 mM dithiothreitol, 0.5 mM each of CTP, ATP, GTP, TTP, incubated for 2 h at 37°C, and denatured at 95°C for 10 minutes.

  • 0% Bacto-tryptone, 0.5% Yeast extract and 1.0% NaCl) with antibiotics at the following concentrations when necessary: ampicillin (Amp) at 50 microgram/ml together with methicillin at 200 microgram/ml, chloramphenicol (Cml) at 25 microgram/ml, kanamycin (Kan) at 10 microgram/ml and tetracycline (Tet) at 10 microgram/ml.


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