Example sentences for: microgram

How can you use “microgram” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • One microgram each of normal and heat-shocked testicular poly(A) RNA was submitted to Genome Systems (now part of IGI) for contract hybridization against their mouse Gene Expression Microarray I (GEM 1 [ 20]).

  • This contrasts with typical mRNA expression analyses where microgram quantities of mRNA are available for direct analysis.

  • One microgram of the total RNA from a single animal was primed with oligo dT and incubated at 70°C for 10 min followed by a brief chill on ice.

  • For RT reactions, one microgram of total RNAs was reverse transcribed from random (dN)6 primers in 20 μl of reaction medium containing 1 mM each deoxynucleoside triphosphate, (dN)6 (2 mM), 20 U of RNAsin, 50 mM Tris-HCl, 75 mM KCl, 5 mM MgCl 2 , and 50 U of Moloney murine leukemia virus reverse transcriptase (Applied Biosystem).

  • One microgram of total RNA was made into a cDNA library by a reverse transcription process using the following conditions: 0.1 ug of oligothymidine, 100 U of reverse transcriptase, 5 ug bovine serum albumin (BSA), 10 U RNAsin (Promega, Madison WI) in a final volume of 10 ul buffer containing 50 mM Tris-HCl (pH 8.3), 75 mM KCl, 3 mM MgCl 2 , 10 mM dithiothreitol, 0.5 mM each of CTP, ATP, GTP, TTP, incubated for 2 h at 37°C, and denatured at 95°C for 10 minutes.


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