Words similar to microglia
Example sentences for: microglia
How can you use “microglia” in a sentence? Here are some example sentences to help you improve your vocabulary:
This agent at concentrations above 10 μM was toxic to microglia.
It is interesting to note, however, that in newborn mice a brief local application of 2 mM domoic acid to the exposed cortical surface led to a massive degeneration of AMPA/KA GluR receptor positive Cajal-Retzius neurons [ 44 ] , severe impairment of cortical neuronal migration in vivo and malformation of the domoic acid-treated neocortex [ 44 ] . Thus, because 1 mM domoic acid was clearly toxic to cerebellar granule neurons both in vitro (our current study) and in vivo [ 44 ] , we selected 1 mM domoic acid for the experiments with rat neonatal microglia reported herein.
Our results clearly confirmed that GluR4 was present in the rat neonatal microglia used in our in vitro studies with domoic acid, demonstrating that the protocol used in our laboratory to isolate microglia did not appear to affect the presence of the AMPA glutamate receptor GluR4 subunits and their colocalization with the CD 11 b/c integrin.
Experimental protocol to determine the effect of domoic acid and LPS on rat neonatal microglia TNF-α expression: RNA isolation and semiquantitative RT-PCR analysis
To isolate rat neonatal microglia, cerebral cortices of 1-2 day-old Sprague-Dawley rats purchased from Harlan (Indianapolis, IN) were surgically removed and placed in cold DMEM + 10% FBS +120 U/ml P and 12 μg/ml S, the meninges carefully removed, and brain tissue minced and dissociated with trypsin-EDTA at 36°C for 3-5 min.
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