Example sentences for: microdissection

How can you use “microdissection” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To overcome the problem of heterogeneity and stromal cell contamination in homogenized tissue specimens, specific cell types were isolated by laser capture microdissection (LCM) prior to analysis.

  • High-molecular weight DNA from frozen tumor and the corresponding peripheral blood specimens, and frozen cells from cell lines was isolated as previously described [ 25 ] . Tumor cells from paraffin-embedded CIN tissue specimens were isolated by laser capture microdissection (Arcturus, Mountain View, CA) after methyl green-staining and the extraction of DNA as described earlier [ 26 ] . Total RNA was isolated from semi-confluent cell cultures using the TRIzol reagent (Life Technologies, Gaithersburg, MD) according to the manufacturer's protocol.

  • For example, in many cancer microarray studies, the actual percentage of cancer cells in a profiled sample may be as low as 30-40% [ 2 ] . Even when using techniques such as laser capture microdissection, which can improve tumor purity [ 3 ] , the relative contribution of cancer cells and non-cancer cells to the overall gene-expression profile remains uncertain.

  • Laser Microdissection (LM)/RT-PCR

  • Microdissection, DNA and RNA isolation and LOH analysis


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