Words similar to microdissected

Example sentences for: microdissected

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Although one group has used unamplified total RNA extracted from ~2 × 10 4microdissected cells for hybridization on 5000 clone membrane-based arrays [ 15 ] , most groups perform RNA amplification for this purpose [ 16 17 18 ] , especially when using high-density slide-based arrays.
The total number of samples studied using LCM and qPCR were 76 (68 samples were microdissected to specifically enrich for cells of epithelial origin, and eight samples were microdissected to obtain tumor-associated stroma).
In addition, eight tumor-associated stromal components (including fibroblasts, endothelial cells and inflammatory cells) were microdissected from primary tumors without known metastases for analysis.
5 wild-type mice along with littermates mutant for the crx gene (which encodes a Paired type homeodomain transcription factor that directly regulates transcription of many photoreceptor-enriched genes); adult mouse retina; and microdissected outer nuclear layer, which consists entirely of rod and cone photoreceptors [ 11 ] . We examined this very large dataset for expression of genes known to be photoreceptor specific (Figure 2).
Briefly, the microdissected cell pellet was incubated overnight in lysis buffer (Qiagen Inc) with proteinase-K at 55°C.