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Example sentences for: meu
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The fact that DAP was able to increase hybridization intensities while MeC and MeU decreased hybridization intensities (Figures 2Dand 2E) is interesting in light of the fact that DAP is a purine incorporated into an RNA strand while MeC and MeU are pyrimidines incorporated into an RNA strand.
Finally, earlier studies have noted that methylation of cytosines (generating MeC) in ribopolynucleotides stabilizes duplexes to the same extent as substitution of thymine (MeU) for uracil [ 28 ] . Thus, although it is still surprising that MeC and MeU decrease hybridization intensities, it is not surprising that the magnitude of the effects generated by MeC and MeU residues are similar.
Although DAP, MeC, and MeU were tolerated by T7 RNA polymerase, incorporation of these analogs into our IVT reaction cocktails reduced the yields of amplified cRNA.
Those probes whose signal intensities were increased at least two-fold are summarized in Table 1. As Table 1shows, for a number of transcripts substitution with MeC or MeU may potentially augment hybridization signals more so than even DAP.
In contrast, both of the modified pyrimidine triphosphates (MeC and MeU) tested resulted in a decrease in overall signal intensities for nearly all of the probes tested when compared against unmodified control samples (Figures 2D& 2E).