Words similar to mecp

Example sentences for: mecp

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Although MeCP2 expression is higher in other tissues such as brain, spleen and lung [ 16 ] , the value gained by the matched pair comparisons of clonal skin fibroblast strains cultured under identical environmental conditions, should overcome the drawbacks of evaluating a tissue with a lower level of expression.
Allele-specific MECP2 transcript analysis
Of the 47 unique genes with increased transcript levels in MeCP2 deficient cells, 32 are known to be expressed in brain (Table 1).
The gel was then equilibrated in transfer buffer for 15 min and transferred to a polyvinylidine difluoride membrane (Novex, San Diego, CA), also equilibrated in transfer buffer for 15 min, for 1 hr at 100 V. The membrane was blocked overnight with 5% non-fat milk in PBS at 4°C, and incubated with a rabbit polyclonal antibody (1:2000) against a peptide (CPRPNREEPVDSRTP) at the C-terminal region of MECP2 (Upstate Biotechnology, Lake Placid, NY) at room temperature for 2 h in a western wash solution (PBS containing 2% non-fat milk, 0.1% Tween 20, and 350 mM NaCl).
Clonal cell lines with the mutant MECP2 allele on the active X chromosome were derived from lymphoblastoid cell lines (LCLs) from four females with clinically diagnosed Rett syndrome and known mutations (T158M, 803delG, R168X) [ 10 ] and 1159del28 (unpublished) using the technique described above.