Words similar to mecp

Example sentences for: mecp

How can you use “mecp” in a sentence? Here are some example sentences to help you improve your vocabulary:

MeCP2 protein expression in fibroblast clones and lymphoblastoid cell lines was determined by immunoblot analysis.
First, redundancy in methyl-CpG binding proteins that recruit transcriptional silencing complexes may mask the effects of MeCP2 deficiency; for example, MBD1, MBD2 or Kaiso, may complement the defect in the cell types we studied.
Absence of DNA contamination was verified by amplifying the 756 bp intron containing region of the MECP2 gene between exons 3 and 4 using exon 3 based primer 5'-AGCCCGTGCAGCCATCAGCC-3' and exon 4 based primer 5'-CTTCCCAGGACTTTTCTCCA-3'.
The gel was then equilibrated in transfer buffer for 15 min and transferred to a polyvinylidine difluoride membrane (Novex, San Diego, CA), also equilibrated in transfer buffer for 15 min, for 1 hr at 100 V. The membrane was blocked overnight with 5% non-fat milk in PBS at 4°C, and incubated with a rabbit polyclonal antibody (1:2000) against a peptide (CPRPNREEPVDSRTP) at the C-terminal region of MECP2 (Upstate Biotechnology, Lake Placid, NY) at room temperature for 2 h in a western wash solution (PBS containing 2% non-fat milk, 0.1% Tween 20, and 350 mM NaCl).
The levels of MECP2 transcripts were virtually identical by real-time quantitative RT-PCR analysis (less than 0.27 cycle thresholds difference) across all eight clones, one wild-type and one mutant from each individual, that were used for oligonucleotide array hybridization (data not shown).