Example sentences for: mecp

How can you use “mecp” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Absence of DNA contamination was verified by amplification of the intron containing region of the MECP2 gene between exons 3 and 4 using exon 3 based primer 5'-AGCCCGTGCAGCCATCAGCC-3' and exon 4 based primer 5'-CTTCCCAGGACTTTTCTCCA-3'.

  • Although MeCP2 expression is higher in other tissues such as brain, spleen and lung [ 16 ] , the value gained by the matched pair comparisons of clonal skin fibroblast strains cultured under identical environmental conditions, should overcome the drawbacks of evaluating a tissue with a lower level of expression.

  • For the 20 different comparisons, we included three mutant-allele expressing clones (T158M, 803delG, R168X) and four completely skewed wild-type MeCP2 expressing LCLs (R106W, R255X, 803delG, 803delG) from different donors.

  • Four cell lines from females with known MECP2 mutations (R106W, R255X, and two with 803delG) but completely skewed X chromosome inactivation with the wild-type MECP2 allele on the active X chromosome were also cultured.

  • To assess MeCP2 expression at the protein level, immunoblot analysis with an antibody against the C-terminal region of MeCP2 was carried out on total cell lysates from all eight fibroblast clones, and from some of the LCLs, that were used for array hybridization (Fig.


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