Words similar to mecp

Example sentences for: mecp

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Immunoblot analysis with MeCP2 antibody
From four original fibroblast samples, we generated clonally derived cell strains that expressed only the mutant MeCP2 or only the wild-type MECP2 allele, but not both.
The gel was then equilibrated in transfer buffer for 15 min and transferred to a polyvinylidine difluoride membrane (Novex, San Diego, CA), also equilibrated in transfer buffer for 15 min, for 1 hr at 100 V. The membrane was blocked overnight with 5% non-fat milk in PBS at 4°C, and incubated with a rabbit polyclonal antibody (1:2000) against a peptide (CPRPNREEPVDSRTP) at the C-terminal region of MECP2 (Upstate Biotechnology, Lake Placid, NY) at room temperature for 2 h in a western wash solution (PBS containing 2% non-fat milk, 0.1% Tween 20, and 350 mM NaCl).
Alternatively, while MeCP2's expression is ubiquitous, other methyl-CpG binding proteins that exist within complexes, MBD1 and MBD2 [ 19 ] or Kaiso [ 20 ] , may reduce the effect of mutations in MECP2 in peripheral organ systems while the lack of such redundancy may lead to a more pronounced phenotype in the brain.
Clonal cell lines with the mutant MECP2 allele on the active X chromosome were derived from lymphoblastoid cell lines (LCLs) from four females with clinically diagnosed Rett syndrome and known mutations (T158M, 803delG, R168X) [ 10 ] and 1159del28 (unpublished) using the technique described above.