Words similar to mec
Example sentences for: mec
How can you use “mec” in a sentence? Here are some example sentences to help you improve your vocabulary:
Although DAP, MeC, and MeU were tolerated by T7 RNA polymerase, incorporation of these analogs into our IVT reaction cocktails reduced the yields of amplified cRNA.
Many studies have shown that a ribopurine rich strand bound to a deoxypyrimidine rich strand has a higher stability than the corresponding all deoxy strands which, in turn, have a higher stability than a deoxypurine rich strand bound to a ribopyrimidine rich strand [ 26 27 ] . These differences also suggest that further studies examining DAP or MeC incorporation into first strand cDNA, followed by formation of a DNA-DNA duplex, may not necessarily show the same effects as incorporation of these modified nucleotides into cRNA.
DNA repair activities may take place at these foci since BRCA1 has been shown to co-localize with ATR in damage-induced foci [ 58 ] . Using elegant methods to test for direct association of DNA damage checkpoint proteins at double strand breaks in vivo , other groups have shown that MEC1 Rad3-HA and LCD1 Rad26-GFP localize to sites of DNA damage [ 59 60 ] . However, MEC1 was required for this re-localization of LCD1-GFP in vivo [ 60 ] . Thus, while re-localization of Rad26/Rad3-related complexes to sites of DNA repair may be evolutionarily conserved, the regulation of these complexes is not.
Similarly, when MeC was fully substituted for cytidine in the IVT reaction cocktail, only a peak corresponding to the MeC was detected (Figure 1D), and this peak was approximately equal in area to that of the cytidine in the control situation.
In budding yeast, LCD1 Rad26physically interacts with MEC1 Rad3and is phosphorylated by MEC1 Rad3following DNA damage [ 36 37 ] . Likewise, human ATRIP Rad26physically interacts with ATR Rad3and also undergoes DNA damage-dependent phosphorylation by ATR Rad3 [ 35 ] . However, Rad26 is required for Rad3 kinase activity in fission yeast [ 18 ] , but LCD1 Rad26is not required for MEC1 Rad3kinase activity [ 38 ] while ATRIP Rad26is required for normal ATR Rad3expression [ 35 ] . Thus, the role of the Rad26-related proteins can differ among organisms.
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