Words similar to mec
Example sentences for: mec
How can you use “mec” in a sentence? Here are some example sentences to help you improve your vocabulary:
Earlier studies have shown that DAP, in DNA fragments, can widen the minor groove, as detected by reactivity towards uranyl nitrate or susceptibility to Dnase cleavage [ 29 ] , and can relieve compressions in the minor groove associated with A-tracts [ 30 ] . In contrast, NMR and molecular modeling studies of an oligonucleotide duplex containing MeC have demonstrated that, while this duplex still has a B-DNA conformation, there are differences in the structural parameters and thermal stability relative to the unmodified duplex [ 31 ] . An earlier study also found, in two closely related octanucleotide duplexes, that although the methylated duplexes retained their B-DNA conformation, different structural and thermal stability effects were seen [ 32 ] . Although these studies have been carried out with DNA-DNA and not DNA-RNA duplexes, it is possible that MeC substitution may have different structural consequences than DAP substitution.
Why do we see differential effects of DAP, MeC, and MeU on hybridization intensities?
In contrast, both of the modified pyrimidine triphosphates (MeC and MeU) tested resulted in a decrease in overall signal intensities for nearly all of the probes tested when compared against unmodified control samples (Figures 2D& 2E).
The fact that DAP was able to increase hybridization intensities while MeC and MeU decreased hybridization intensities (Figures 2Dand 2E) is interesting in light of the fact that DAP is a purine incorporated into an RNA strand while MeC and MeU are pyrimidines incorporated into an RNA strand.
Similarly, when MeC was fully substituted for cytidine in the IVT reaction cocktail, only a peak corresponding to the MeC was detected (Figure 1D), and this peak was approximately equal in area to that of the cytidine in the control situation.
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