Example sentences for: methylation-sensitive

How can you use “methylation-sensitive” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • By performing PCR amplification across this region after cutting with the methylation-sensitive enzyme Hpa II, the relative amounts of the methylated AR alleles can be quantitatively determined with a high degree of accuracy; variance on repeated assays is approximately 4% [ 12].

  • Considering the varying definitions of CpG islands (especially the minimum length of CpG-rich region), this number is in close agreement with the estimate of 45,000 obtained by Antequera and Bird [ 28] using methylation-sensitive restriction enzymes.

  • Current methods to detect tumor clonality include methylation-sensitive restriction enzyme digestions and detection of X chromosome polymorphisms, neither of which give an overall genomic assessment of tumors [ 2].

  • Genomic DNA (approximately 200 ng) was digested overnight using a methylation-sensitive restriction enzyme HpaII, as per the manufacturer's instructions (Gibco BRL, Rockville, MD, USA).


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