Words similar to mes
Example sentences for: mes
How can you use “mes” in a sentence? Here are some example sentences to help you improve your vocabulary:
BCIP = 5-bromo-4-chloroindol-3-yl phosphate; cDNA = complementary DNA; EGTA = (ethylene-bis [oxyethylenenitrilo])tetraacetic acid; CHTN = Cooperative Human Tissue Network; ELISA = enzyme-linked immunosorbent assay; MAP = microtubule associated protein; MES = 2-( N -morpholino)-ethane sulfonic acid; NBT = nitro blue tetrazolium; PBS = phosphate-buffered saline; PC-tubulin = MAP-free phosphocellulose-purified tubulin; PCR = polymerase chain reaction; PIPES = piperazine- N,N '-bis(2-ethanesulfonic acid); PVDF = poly(vinylidene difluoride).
These cells were treated with 10 μM Monensin and 10 μM Nigericine and equilibrated for 2 min with MES (4-morpholineethanesulfonic acid) calibration buffer with pHs from 4.0 to 7.0 prior to image acquisition.
The procedure for arginine-Sepharose purification of tubulin was presented in detail by Lacey and Snowdon [ 42 ] . An enriched tubulin fraction was collected in high-salt MES buffer (2.
9 M NaCl, 60 mM NaH 2 PO 4 , 6 mM EDTA, 0.01% Tween-20) at 25°C followed by stringent wash buffer (100 mM methyl ethyl sulfonate (MES), 100 mM NaCl, 0.01% Tween-20) at 50°C in a fluidics station (Affymetrix).
The supernatant was then loaded on a 2 ml arginine-Sepharose column equilibrated with the MES buffer above without Triton X-100.
Loading...