Words similar to membrane-localization
Example sentences for: membrane-localization
How can you use “membrane-localization” in a sentence? Here are some example sentences to help you improve your vocabulary:
, to a membrane-localization tag) improve the function of mutant versions of Boi1-PH that are impaired in the ability to bind PIP 2 ? One possibility is that each of these mutant versions of Boi1 retains at least a slight affinity for PIP 2 and that the myristoyl group causes these mutant PH domains to be apposed close enough to the lipid bilayer to enable the residual PIP 2 -binding activity to now be sufficient for binding to PIP 2 . We speculate that, just as binding to PS may serve to position the PH domain of wild-type Boi1 near the plasma membrane in a manner that facilitates binding to PIP 2 , the myristoyl group may help to situate the PH domain of the mutant versions of Boi1-PH at the plasma membrane in a manner that facilitates binding to PIP 2 .
, to serve as a membrane-localization tag) [ 13 14 ] . In other proteins, the binding of PH domains to phospholipids appears to be important for allosteric or other types of regulation [ 15 16 ] .
If this were the only role for such binding, then attachment to a membrane-localization tag might be able to restore function to versions of Boi1 that are impaired in the ability to bind phospholipid.
One type of membrane-localization tag is the myristoyl group, which is attached to the glycine residue of proteins, such as S. cerevisiae Gpa1 (the α subunit of the pheromone-responsive G protein) [ 18 ] , that contain at their N-termini the sequence MGXXXS/T (where "X" can be any amino acid) [ 19 ] . Thus, we investigated whether fusion to the sequence MGCTVS, which is present at the N-terminus of Gpa1 and which we will henceforth refer to as "Myr", could restore function to the mutant versions of Boi1 that are impaired in the ability to bind phospholipid.
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