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Example sentences for: mdah

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In parallel, MDAH cells were also treated with tetracycline and transferred to suspension culture.
MDAH human fibroblasts from Li Fraumeni patients (p53-negative), and the same cells stably transfected with a tetracycline-regulated p53 expression vector (TR9-7) [ 42 ] , were obtained from W.R.
In conclusion, MDAH cells (p53-negative), 10T1/2 cells (p53-positive), and TR9-7 cells (high or low levels of p53) all exhibited similarly increased expression levels of PTEN in response to the disruption of cell-matrix interactions.
Although clearly induced in both cell lines, the kinetics of PTEN induction in 10T1/2 and MDAH cells were somewhat different at later time points; i.e., there was a slight reduction of PTEN mRNA and protein in MDAH cells at 36 hours, possibly indicating some cell type-specific differences.
After having established that PTEN protein phosphatase activity could be determined specifically, we transferred MDAH cells to suspension culture conditions and measured PTEN activity at various times afterwards.