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Example sentences for: mdck

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One explanation for the decrease in AA is that U0126 inhibits [Ca 2+] i mobilization in response to ATP or IONO, thereby preventing translocation of cPLA 2 . In chick ventricular myocytes, inhibition of MEK1/ERK by PD98059 inhibits zinterol-mediated AA release, but also inhibits zinterol-induced stimulation of [Ca 2+] i cycling in electrically stimulated cells [ 33 ] . In MDCK cells, extracellular ATP acts via P 2Y2 receptors to elicit an IP 3 -mediated [Ca 2+] i increase [ 34 ] and IONO acts to increase [Ca 2+] i by permeabilizing cell membranes to Ca 2+.
To study the role of the MEK1/ERK pathway in cPLA 2 activation, quiesced MDCK cells were treated with the MEK1 inhibitor U0126, and the effect on AA release, ERK activation, and cPLA 2 gel shift determined (Fig.
MDCK cells obtained from ATCC were cultured in DMEM containing 10% FBS, 100 U/ml penicillin, 100 μg/ml streptomycin, 0.292 mg/ml glutamine (growth medium) in 5% CO 2 at 37°C.
Water permeability was measured as the net fluid movement driven by an osmotic gradient across intact monolayers of adenovirus-infected MDCK cells, by methods similar to those reported previously [ 27 29 ] . Cells were seeded onto Transwell filters (Costar, 1 cm 2, 0.4 μm pore size) at a density of 1.5 × 10 5cells per well.
The protocol for determining AA release is essentially as described [ 15 21 ] . MDCK cells stably expressing EGFP-cPLA 2 were plated in 12-well plates at 5 × 10 3cells/cm 2and incubated in growth medium overnight.