Words similar to mdck
Example sentences for: mdck
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MDCK cells grown on MatTek plates were quiesced overnight in serum-free medium, washed with HHBSS containing 1 mM probenecid and incubated with 5 μM Fura2-AM (Calbiochem) in HHBSS, 1 mM probenecid, and 1% DMSO for 45 min at 37°C.
Treatment of MDCK cells with 30 μM PD098059, a less potent inhibitor of MEK [ 28 29 ] , resulted in a ~50% reduction in AA release in response to 100 μM ATP, 1 μM IONO, and 10 μM IONO (data not shown).
1 cells, without constitutively active ERKs, AA release is delayed after [Ca 2+] i mobilization, and is temporally correlated with ERK activation [ 40 ] , whereas in MDCK cells with constitutively active ERKs, AA release is rapid, with significant AA release measured 30 s after [Ca 2+] i increase [ 15 ] . This temporal correlation between AA release and ERK activation has also been reported in CHO cells in response to PAF stimulation [ 35 41 ] . These results support the observations made here that ERK activity is required for phospholipid hydrolysis independently of cPLA 2 translocation.
MDCK cells obtained from ATCC were cultured in DMEM containing 10% FBS, 100 U/ml penicillin, 100 μg/ml streptomycin, 0.292 mg/ml glutamine (growth medium) in 5% CO 2 at 37°C.
Water permeability was measured as the net fluid movement driven by an osmotic gradient across intact monolayers of adenovirus-infected MDCK cells, by methods similar to those reported previously [ 27 29 ] . Cells were seeded onto Transwell filters (Costar, 1 cm 2, 0.4 μm pore size) at a density of 1.5 × 10 5cells per well.
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