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Example sentences for: mdck

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MDCK cells were induced to express AQP1 by infection with replication-deficient adenovirus (see Materials and Methods).
2 , it is necessary to over-express the enzyme several fold as previously reported [ 16 ] . Cytosolic PLA 2 has been shown to mediate Ca 2+-induced AA release in MDCK cells treated with ATP and IONO in experiments using the group IV cPLA 2 α-specific inhibitor pyrrolidine-1 [ 27 ] . To measure cPLA 2 mediated AA release, EGFP-cPLA 2 -transfected MDCK cells labeled with [ 3H]-AA were incubated with 0.3, 1 or 10 μM U0126 for 15 min prior to stimulation with 100 μM ATP, 1 μM IONO, or 10 μM IONO.
To study the role of the MEK1/ERK pathway in cPLA 2 activation, quiesced MDCK cells were treated with the MEK1 inhibitor U0126, and the effect on AA release, ERK activation, and cPLA 2 gel shift determined (Fig.
MDCK Madin-Darby Canine Kidney cell
Thus, in MDCK cells, MEK1 inhibition significantly reduces the ability of cPLA 2 to hydrolyze AA from membrane phospholipids.