Words similar to mdck

Example sentences for: mdck

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ERK was found to be constitutively phosphorylated in our study using MDCK cells from ATCC, in contrast to what has been reported previously for MDCK-D 1 , a subclone of MDCK selected for adrenergic receptor expression [ 39 40 ] . However, a side-by-side comparison of MDCK cells from ATCC and the MDCK-D 1 subclone (kindly provided by Dr. Paul Insel, UCSD) demonstrated constitutive activation of ERKs when both are grown at low density (not shown).
The protocol for determining AA release is essentially as described [ 15 21 ] . MDCK cells stably expressing EGFP-cPLA 2 were plated in 12-well plates at 5 × 10 3cells/cm 2and incubated in growth medium overnight.
MDCK cells grown on MatTek plates were quiesced overnight in serum-free medium, washed with HHBSS containing 1 mM probenecid and incubated with 5 μM Fura2-AM (Calbiochem) in HHBSS, 1 mM probenecid, and 1% DMSO for 45 min at 37°C.
MDCK cells transfected with empty virus showed no augmentation of transepithelial water movement, confirming previous work that showed water does not flow across tight junctions in MDCK confluent layers [ 16 ] . Cells transfected with empty virus also showed no effect of TEA treatment.
MDCK cells were co-transfected with EYFP-cPLA 2 and a cPLA 2 with a S505A mutation fused to ECFP (ECFP-cPLA 2 S505A).