Words similar to mdck

Example sentences for: mdck

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TEA inhibition of water flux in AQP1-expressing MDCK cells
MDCK cells obtained from ATCC were cultured in DMEM containing 10% FBS, 100 U/ml penicillin, 100 μg/ml streptomycin, 0.292 mg/ml glutamine (growth medium) in 5% CO 2 at 37°C.
Water permeability was measured as the net fluid movement driven by an osmotic gradient across intact monolayers of adenovirus-infected MDCK cells, by methods similar to those reported previously [ 27 29 ] . Cells were seeded onto Transwell filters (Costar, 1 cm 2, 0.4 μm pore size) at a density of 1.5 × 10 5cells per well.
ERK was found to be constitutively phosphorylated in our study using MDCK cells from ATCC, in contrast to what has been reported previously for MDCK-D 1 , a subclone of MDCK selected for adrenergic receptor expression [ 39 40 ] . However, a side-by-side comparison of MDCK cells from ATCC and the MDCK-D 1 subclone (kindly provided by Dr. Paul Insel, UCSD) demonstrated constitutive activation of ERKs when both are grown at low density (not shown).
The immunoblots demonstrate that 30 min treatment in 25 ng/ml anisomycin resulted in phosphorylation of p38 in unstimulated MDCK cells and in cells treated with ATP or IONO.