Words similar to mdck
Example sentences for: mdck
How can you use “mdck” in a sentence? Here are some example sentences to help you improve your vocabulary:
To better understand the regulation of cPLA 2 by the MEK1/ERK pathway and Ca 2+, we investigated the effect of MEK inhibitors on AA release, cPLA 2 phosphorylation of Ser 505, cPLA 2 translocation kinetics, and [Ca 2+] i increase in Madin-Darby canine kidney (MDCK) cells.
MDCK cells were induced to express AQP1 by infection with replication-deficient adenovirus (see Materials and Methods).
MDCK cells transfected with empty virus showed no augmentation of transepithelial water movement, confirming previous work that showed water does not flow across tight junctions in MDCK confluent layers [ 16 ] . Cells transfected with empty virus also showed no effect of TEA treatment.
TEA inhibition of water flux in AQP1-expressing MDCK cells
1 cells, without constitutively active ERKs, AA release is delayed after [Ca 2+] i mobilization, and is temporally correlated with ERK activation [ 40 ] , whereas in MDCK cells with constitutively active ERKs, AA release is rapid, with significant AA release measured 30 s after [Ca 2+] i increase [ 15 ] . This temporal correlation between AA release and ERK activation has also been reported in CHO cells in response to PAF stimulation [ 35 41 ] . These results support the observations made here that ERK activity is required for phospholipid hydrolysis independently of cPLA 2 translocation.