Words similar to lif
Example sentences for: lif
How can you use “lif” in a sentence? Here are some example sentences to help you improve your vocabulary:
The ligands ciliary neurotrophic factor (CNTF), cardiotrophin-1 (CT-1) and a novel neurotrophin set, known alternatively as NNT-1/BSF3 and CLF/CLC, also function through the high affinity LIFR:gp130 heterodimeric receptor but require a third ligand-binding but non-signaling subunit [ 1 2 3 4 ] . Oncostatin M (OSM) utilizes LIFR:gp130 as an alternative receptor complex for OSM signaling in humans [ 5 ] , but not in mice [ 6 ] . Thus, in mice LIFR:gp130 is obligatory for signaling induced by LIF, CNTF, CT-1 and CLF/CLC.
Adult mouse LIF over expression mediated by transfer of LIF over expressing hematopoietic cells results in a lethal, multi-systemic phenotype that includes small adrenals with a loss of the innermost cortical layer [ 28 ] . Pituitary specific LIF over expression leads to a Cushing's Syndrome-like condition of GC hyperactivity [ 29 30 ] . LIF null adult mice have normal or slightly reduced basal ACTH and GC concentrations, and are unable to respond to acute stress by increased ACTH.
Cells were plated on thirteen 10 cm 2tissue culture dishes and grown for two days in ES cell medium supplemented with LIF.
LIF and LIFR are functionally expressed in rodent tissues that are central to GC production: the hypothalamus [ 8 ] and pituitary [ 9 10 11 12 ] . LIF works in synergy with corticotropin-releasing hormone (CRH) in the hypothalamus to increase pituitary pro-opiomelanocortin (POMC) and, ultimately, pituitary adrenocorticotrophin hormone (ACTH) [ 9 11 13 14 15 16 ] . LIF also stimulates POMC gene expression directly in the pituitary [ 9 11 15 17 ] . LIF up-regulates pituitary prohormone convertase (PC1).
100 μg of pHDMCCre8 was introduced into 5 × 10 7ES cells by electroporation using 400 v/500μf/resistance setting 8. 1/100,000 of the total electroporated cell population was plated per 10 cm 2tissue culture dish in ES cell medium supplemented with LIF and grown until individual colonies could be collected.
Loading...