Example sentences for: lgl
How can you use “lgl” in a sentence? Here are some example sentences to help you improve your vocabulary:
In order to identify the differentially expressed genes in large granular lymphocytic (LGL) leukemia, we performed microarray analysis using the UniGEM-V microarray from IncyteGenomics and the HU6800 oligonucleotide array from Affymetrix.
Similarly an anti-apoptotic gene A20 is also over-expressed in leukemic LGL, but protein expression was absent when Western blots were performed with monoclonal antibodies raised against the amino acid sequence derived from A20 (data not shown).
Briefly, total RNA isolated from normal PBMC and leukemic LGL were DNase-treated and purified with a Qiagen kit (Valencia, CA).
The results of all the experiments did not confirm the over-expression of PAC-1 . Therefore, to obtain more information about the structure of the PAC-1 related genes in leukemic LGL, we screened an LGL leukemia cDNA library using a 1.2 kb PAC-1 cDNA fragment and identified similar genes which are different forms of PAC-1 (GenBank Accession #AF331843, the other sequence is not deposited).
RT-PCR was performed using total RNA from leukemic LGL and specific probes designed to amplify full-length PAC-1.