Words similar to ks
Example sentences for: ks
How can you use “ks” in a sentence? Here are some example sentences to help you improve your vocabulary:
The CaCET1 gene was disrupted by insertion of a UAU1 cassette [ 16 ] . We first constructed plasmid pKS-5'3'CaCET1, in which a 665-bp PCR fragment derived from the 5' end of the CaCET1 gene (from nucleotides -50 to +615 of the open reading frame, with the A residue of the ATG translation start codon defined as position +1) was cloned between the Kpn I and Xba I sites of pBluescript KS +and a 720-bp fragment extending from position +1518 of the 1560-nt CaCET1 coding sequence into the 3' flanking genomic region was inserted between the Sac I and Sac II sites of pBluescript KS +The 3.8-kbp UAU1 gene was excised from pBME101 with Xba I and Sac II and inserted between the Xba I and Sac II sites of pKS-5'3'CaCET1 to yield pCaCET1::UAU1.
Each of these PCR products was cloned individually into the Eco RV site of pBluescript KS+, and then restriction digested with Eco RI and Not I (first-set fragment) or Not I and Pst I (second-set fragment).
Each PCR product was gel-purified and cloned into the Eco RV site of pBluescript KS+ (Stratagene).
Prehybridization was performed at 67°C for two hours in a seal-a-meal bag (Dazey Corporation, Industrial Airport, KS) with hybridization solution (0.
The resulting fragment was subcloned into the same sites of pBlueScript II KS (+).
Loading...