Words similar to kinase-inactive

Example sentences for: kinase-inactive

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Initially, we tested Chinese hamster ovary (CHO) cells that had been stably transfected with expression vectors for kinase-inactive or wild type IGF-I receptors [ 53 ] . The parental CHO cell line expresses ~50,000 endogenous IGF-IRs, the transfected wild-type (CHO-WT) cell line expresses ~250,000 IGF-IRs, and the kinase-defective (CHO-MK) cell line expresses ~250,000 mutant IGF-IRs.
Two forms of Btk:ER were generated, one using wild-type (WT) Btk and the other using a kinase-inactive (K430E) mutant of Btk as a negative control (Fig 1A).
This interaction was not dependent on the activity of either kinase, as co-immunoprecipitation was observed with either wild-type (WT) or kinase-inactive versions of the two proteins.
This was not observed in cells transfected with kinase-inactive Fyn, and the increase in kinase activity on enolase appeared to be due to Rlk rather than Fyn, since we barely detect phosphorylation of enolase when WT Fyn is co-expressed with kinase-inactive Rlk [ 19].
His6-T7-Net1N/GST-T7-Cdc14 complex was assembled on anti-GST beads, and treated with either active or N172A kinase-inactive Plx1 (Polo-like kinase from Xenopus ; [ 24 ] ) in the presence of ATP.