Words similar to kanmx

Example sentences for: kanmx

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The strain is a derivative of VL6-48 with the ura3-52 gene replaced by the KanMX cassette [ 15 ] . Spheroplasts were generated as described previously [ 29 ] . Agarose plugs (60 ul) containing approximately 2 ug of high molecular weight DNA were prepared from normal human fibroblasts MRC-5 (ATCC) or from liver cells of the Tg.AC mouse [ 11 ] . Linearized TAR cloning vector (1 ug) was added to the plugs, treated with agarase, and mixed with yeast spheroplasts.
The pct1::kanMX cassette was PCR-amplified from the pPCT1Δ plasmid using primers LI and L4.
Similarly, half of the Leu +haploids derived from a pce +/ pce1::kanMX strain containing the pce1 +plasmid were resistant to G418.
Genomic DNA was prepared from individual isolates and the integration of the pct1::kanMX or pce1::kanMX cassettes into the correct locus was tested by PCR using diagnostic primers.
pct1 +/ pct1::kanMX or pce1 +/ pce1::kanMX strains containing the control LEU2 plasmid vector lacking an insert were G418-resistant.