Example sentences for: kanamycin

How can you use “kanamycin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The plasmids containing the various group I constructs were transformed into competent C600ThyA::Kan Rcells and grown in LB with kanamycin overnight.

  • Plasmids pT2TFXK and pT2TX3K confer resistance to TFX, tetracycline, and kanamycin with pT2TFXK also providing TFX production to a host strain [ 20 ] . Since strains F2/5 and F2/5(pT2TX3K) produced no zones of inhibition against ANU794 or ANU794(pT2TX3K) (data not shown), F2/5(pT2TFXK) is producing TFX.

  • Mutazyme (Stratagene, La Jolla, CA) was used to amplify PcrG for cloning into pBAD18 [ 33 ] . The ligation reaction was electroporated into lcrG Y. pestis (which is temperature sensitive due to its Ca 2+-blind phenotype) and the cells were plated on TMH medium [ 29 ] containing arabinose, Ca 2+, kanamycin and carbenicillin.

  • Top 10 F' E. coli (Stratagene) were transformed with 1 μL of the transposition reaction and plated on LB agar with either ampicillin (100 μg/mL) and kanamycin (50 μg/mL) to recover transposed clones, or ampicillin (100 μg/mL) alone to establish the transposition efficiency.

  • Dilutions of this culture were plated on LB agar containing 50 μg kanamycin/mL to give well-separated colonies.


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