Example sentences for: kanamycin

How can you use “kanamycin” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • To determine the proportion of active promoters in the library, a portion of the transformation mixture was spread on medium containing hygromycin B (all recipients), and a portion was spread on medium containing kanamycin (recipients with an active promoter).

  • The plasmids containing the various group I constructs were transformed into competent C600ThyA::Kan Rcells and grown in LB with kanamycin overnight.

  • The E. coli (pYUB178::H37Rv) library was grown in Luria broth (LB) (Difco Laboratories, Detroit, Mich.) containing 50 μg kanamycin/mL (Sigma Chemical Company, St. Louis, Mo.)

  • Ampicillin, kanamycin, α-ketoglutarate, shikimate, and amino acids were purchased from Sigma Chemical Company, DTT was from Research Organics, DEAE-cellulose was from Whatman, hydroxylapatite was from Bio-Rad.

  • The cultures are centrifuged at 2500 × g at 4°C for 20 minutes, the supernatant is discarded, and the bacterial pellet is resuspended in 400 μl of 2x YT-AK (ampicillin and kanamycin) medium and is incubated overnight at 37°C.


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