Words similar to kanamycin-resistant
Example sentences for: kanamycin-resistant
How can you use “kanamycin-resistant” in a sentence? Here are some example sentences to help you improve your vocabulary:
To differentiate between promoters dependent on the regulator and other promoters, plasmid DNA from a pool of the kanamycin-resistant transformants is transformed into bacteria that do not express the transcriptional regulator.
To differentiate between σ E-dependent and -independent promoters, approximately 40,000 kanamycin-resistant transformants were pooled, and plasmid DNA was isolated and electroporated into M. smegmatis LR222::Δ sigE bacteria, in which the wild type copy of the sigE gene had been disrupted with the gentamicin-resistance gene.
When the initial pool of kanamycin-resistant colonies sprayed with catechol, the colonies were light to dark yellow indicating a wide range of XylE activity.
However, repeated attempts to electroporate the 4 kb plasmid into M. smegmatis LR222 did not generate any stable kanamycin-resistant transformants (data not shown).
Electrocompetent M. smegmatis LR222Δ sigE cells were electroporated with approximately 1 μg plasmid DNA from the pool of kanamycin-resistant transformants, and recipients were plated on TSA with hygromycin B (50 μg/ml).
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