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Example sentences for: junctions

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Previous studies have shown that E. coli DNA topoisomerase I cleavage of single-stranded DNA occurs with selectivity for sites with the C nucleotide base at the - 4 position [ 9 10 ] and that the enzyme preferentially cleaves at junctions of double-stranded and single-stranded DNA [ 8 ] . Several different 5'-end labeled substrates were prepared and used in cleavage assays to compare the cleavage sites selected by Top67 versus topoisomerase I. The results showed that with single-stranded substrates, Top67 also preferred cleavage sites with a C nucleotide base at the -4 position as reported for most of the type IA topoisomerases [ 12 ] . There were some differences from topoisomerase I in the relative distribution of cleavage products among the potential cleavage sites (Figure 4a,4b).
For the adjacent AA sequences, nearly 75% occur in loops, while approximately 12% of the AA sequences occur in helices, another 12% occur at the 3'loop-5'helix junction, and less than 5% occur in 3'helix-5'loop junctions.
The splice junctions follow the GT/AG rule (Table 1).
In this manner, the entire coding region and all splice junctions of Tlr4 could be covered with a total of 14 sequencing reads.