Example sentences for: isopropanol

How can you use “isopropanol” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • [ 34 ] . For protein detection, the gels were sequentially incubated in 0.5% Coomassie Brilliant Blue in 50% methanol/10% acetic acid and in 10% methanol/10% isopropanol.

  • Alternatives such as ethanol or isopropanol precipitations were explored, but for the chosen PCR conditions, produced less reliable results and appeared to cause the co-precipitation of non-DNA material that interfered with downstream operations.

  • DNA was extracted with phenol/chloroform/isoamyl alcohol (25:24:1) solution and precipitated with an equal volume of isopropanol.

  • The purple formazan product indicative of viable cells was then dissolved by adding 250 μl acidified isopropanol (95% isopropanol; 5% 2N HCl), and 200 μl aliquots were collected.

  • Samples were mixed by vortexing and allowed to stand at room temperature for 2-5 minutes more, then centrifuged for 15 min at 10,000 g . The upper aqueous layer was removed to a clean tube and RNA was precipitated with an equal volume of isopropanol.


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