Words similar to isoform-specific
Example sentences for: isoform-specific
How can you use “isoform-specific” in a sentence? Here are some example sentences to help you improve your vocabulary:
LLWO2F cell extracts (50 ug) were immunoprecipitated with approximately 1 ug of isoform-specific rabbit antiserum to PP1α as previously described [ 38 ] . Immunoprecipitation with 1 ug of normal rabbit serum served as a negative control.
Compartmentation of PKC isoforms has been suggested as a mechanism for targeting selectivity near their physiological substrates [ 9 10 ] . Consistent with this theme of selectivity, work with isoform-specific antibodies demonstrated that PKC isoforms have distinct subcellular locations [ 11 ] .
We discuss the potential significance of these different modes of ER interaction with N-CoR in terms of known isoform-specific behaviors.
Both ERs enhance transcription from genes with classical estrogen response elements (EREs), but ERα requires less ligand to obtain maximal activation than ERβ [ 25 26 ] . Likewise, both ERs suppress the activity of the TNFα promoter in response to estrogens, but ERβ is a more potent repressor than ERα [ 27 ] . However, some of the most striking isoform-specific differences in gene regulation are observed at promoters, such as that of cyclin D1, which contain AP-1 sites or related cyclic AMP response elements (CREs).
The NR family contains two related ERs (ERα and ERβ) that conform to the typical three domain NR structure and share extensive sequence homology in the DBD and LBD region [ 20 21 ] . Analysis of the function of the individual ERs in mouse knockout models suggests that the major proliferative effects of estrogen are mediated by ERα and not by ERβ, which seems to play an inhibitory role in proliferation in some studies [ 22 23 ] . The ligand-binding properties of the ERs are different, with ERβ often exhibiting stronger binding to plant-derived phytoestrogens [ 24 ] . More importantly, the ERs exhibit isoform-specific effects on gene expression.
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