Words similar to intergenic
Example sentences for: intergenic
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Nontranscribed (intergenic) and transcribed (UTR and intron) non-coding sequences were analyzed together [ 44].
Published methods include enterobacterial repetitive intergenic consensus sequence-PCR (ERIC-PCR), and repetitive intergenic palindromic sequence-PCR (REP-PCR) [ 2 3 ] ; random amplified polymorphic DNA-PCR (RAPD-PCR) or arbitrary primed-PCR (AP-PCR) [ 4 5 ] ; and restriction fragment length polymorphism-PCR (RFLP-PCR) of the omp 2 locus [ 6 7 ] . ERIC-PCR and RAPD-PCR are both affected by assay conditions and environmental effects during the amplification process [ 8 9 10 ] . Although the results are highly reproducible within a laboratory, laboratory-to-laboratory reproducibility has been problematic and thus makes the universal application of these methods unlikely (compare [ 2 ] and [ 3 ] ). RFLP-PCR of the omp 2 locus is not constrained by issues of reproducibility and has been useful for the differentiation of Brucella species [ 6 ] and for differentiation among isolates from marine mammal hosts [ 7 ] . However, as a tool for the epidemiology of brucellosis in livestock, this technique is limited by the low rate of natural sequence divergence within the locus at the biovar level.
We used the translated protein sequences from these intergenic ORFs and analyzed them using InterPro Scan via EXPASY.
The CG5245 -like gene in D. pseudoobscura and the CG8319 -like gene in D. willistoni both are located in the same intergenic region between the Arc42 and PK92B genes, but result from independent events since they involve different ancestral sequences and occur on opposite strands in this intergenic region.
Whole pseudogenes were found by searching for matches to SWISS-PROT protein sequences in intergenic DNA, and are usually characterized by frameshift mutations or early stop codons that prevent normal transcription or translation [ 18, 19].
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