Example sentences for: immobilon-

How can you use “immobilon-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In male and female rats from experiments 1 and 2, CALB and COX-2 levels in the frontal cortex and hippocampus (CALB only) were determined using Western blot analysis, as reported elsewhere [ 49 55 ] . In brief, homogenized samples (30 μg per lane for frontal cortex and hippocampus- by treatment) were resolved on 14% Tri-Glycine gels and transferred onto Millipore Immobilon-P membranes (Millipore, Bedford, MA, USA) by electroblotting.

  • Total cellular protein was separated on 4-20% Tris-glycine gels (Novex, Inc.) and transferred to a polvinylidene difluoride (PVDF) membranes (Immobilon-P transfer membranes; Millipore Corp.) overnight at 0.08 A. Following the transfer, blots were blocked with 5% non-fat dry milk in 50 ml of TNE 50 (100 mM Tris-Cl [pH 8.0], 50 mM NaCl, 1 mM EDTA) plus 0.1% NP-40.

  • Proteins resolved by SDS-PAGE were transferred to Immobilon-P membranes (Millipore Corp., Bedford, MA) using carbonate transfer buffer (pH 9.9) [ 5 ] . Specific proteins were visualized using rabbit polyclonal antibodies specific for glutathione S-transferase (GST)-tagged LcrG (α-LcrG) [ 4 ] , His-tagged LcrV (α-LcrV) [ 4 ] , YopM (α-YopM) [ 36 ] , GST-tagged LcrQ (α-LcrQ) [ 16 ] , YopN (aka LcrE) (α-LcrE [ 4 ] ), His-tagged YopD (α-YopD) [ 16 ] , and YopE (α-YopE; gift from S. C. Straley, University of Kentucky, Lexington, KY.

  • The samples from the coimmunoprecipitations described above were analyzed by electrophoresis on an SDS-12% polyacrylamide gel for 3 h at 160 V. The gel was equilibrated in transfer buffer (33 mM Tris base, 192 mM glycine, 20% methanol) for 15 min before transfer of proteins to Immobilon-P membrane (Millipore Corp.).

  • Supernatant (4:1 in denaturing loading buffer, boiled 5 minutes) was loaded, separated on 10% denaturing SDS-polyacrylamide gels, and transferred to Immobilon-P membranes.


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