Words similar to immobilization
Example sentences for: immobilization
How can you use “immobilization” in a sentence? Here are some example sentences to help you improve your vocabulary:
In contrast, when C6 cells were cultured directly on streptavidin-coated wells, on which Ad5.CMV-LacZ had been immobilized, considerable amounts of infection were seen when 2.5 × 10 6viral particles per well and greater were added to wells at the immobilization step.
Remarkably, preincubation with the MEK1 inhibitor PD98059 resulted in a reduced cytoskeletal immobilization and tyrosine phosphorylation of cellular proteins in response to stimulation with mature TGFβ 1 . This finding is in agreement with other observations that MEK1-mediated signal transduction is involved in cytoskeletal remodeling and integrin engagement [ 70 71 ] .
This immobilization process is efficient enough to be iterated, so that DNA sequences specifying multiple amino acids can be routed to the appropriate miniature resin-filled columns during each step.
Clearly, our oligonucleotide immobilization protocol should be sufficient to sustain routine microarray hybridization and wash procedures, which are much less stringent than the overnight wash at 42°C with 0.2% SDS and no salt.
This result is in contrast with our previous study, which showed that greater than 95% of biotinylated Ad5.CMV-LacZ, prepared under the same conditions, can be readily captured on streptavidin-coated microbeads [ 10 ] . Thus, the relatively low immobilization efficiency of biotinylated Ad5.CMV-LacZ on the surface of streptavidin-coated wells is not attributable to the absence of accessible biotin moieties on the surfaces of a fraction of viral particles or the presence of unreacted biotinylation reagent in the biotinylated Ad5.CMV-LacZ preparations.
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