Words similar to immobilized
Example sentences for: immobilized
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The resulting biotinylated adenoviral vectors were incubated at 25°C for 2 h with gentle shaking in polystyrene wells, on which streptavidin is covalently immobilized (well diameter, 0.64 cm; Reacti-Bind Streptavidin Coated Polystyrene Strip Plates; Pierce Chemical) (5 × 10 6viral particles per well).
Consistent with our microarray results on CCR5 up-regulation, experiments performed in peripheral blood mononuclear cells (PBMCs) with soluble Tat has shown selective entry and replication of CCR5 virus into cells [ 27 28 ] . Up-regulation of HIV-1 coreceptor by Tat has also been reported, where a synthetic Tat protein that was immobilized on a solid substrate, up-regulated the surface expression of the chemokine receptors in purified populations of primary resting CD4+ T cells.
In this strategy, adenoviral particles are immobilized, tightly and stably, on solid surfaces (virus-coated solid surfaces), while maintaining their natural properties, including infectivity.
In our study, LPS and lipid A samples from P. aeruginosa 9027 and B. cepacia 25416 were immobilized on a hydrophobic HPA sensor chip (BR-1000-30, Biacore AB) that was found, in our preliminary experiments, to provide an optimal matrix for LPS and lipid A. The surface of HPA sensor chips is composed of long-chain alkanethiol molecules that form a flat, quasi-crystalline hydrophobic layer that facilitates hydrophobic adsorption of liposomes or micelles on a user-defined polar lipid monolayer.
cDNA probes were competitively hybridized to a human UniGEM-V cDNA microarray containing approximately 7075 immobilized cDNA fragments (4107 for known genes and 2968 for ESTs).