Words similar to icka
Example sentences for: icka
How can you use “icka” in a sentence? Here are some example sentences to help you improve your vocabulary:
Immune complex kinase assay (ICKA)
In unstimulated clones expressing the (K395A/3F)Syk molecule, the possibility existed that the intense band observed in the ICKA (Figure 3Dlanes 4, 7, and 10) was not due to increased Syk activity, but rather to the ability of the background levels of active endogenous Syk to preferentially phosphorylate the (K395A/3F)Syk molecule due to possible changes in its three-dimensional structure.
BCR-induced interaction between Cbl and Syk increases the susceptibility of Syk to proteolysis [ 55 ] , possibly through enhancement of ubiquitination [ 56 ] . Expression of a dominant-negative SHP-1 phosphatase in the mature murine B cell line K46 resulted in an increase in BCR-induced Syk kinase activity as measured by C3b phosphorylation in an ICKA and enhanced BCR-induced Ca 2+fluxes [ 57 ] . It remains to be determined if interference with any of these known regulatory proteins is responsible for the dominant-positive effect.
To examine the ability of Syk from cells that expressed (K395A/3F)Syk to phosphorylate an exogenous Syk target, c3b-GST was added to the Syk ICKA (Figure 4, lower panels).
IgM - immunoglobulin of the M isotype; ova - chicken ovalbumin; PY - phosphotyrosine; PLCγ2 - phospholipase C γ2; anti - antibody or antiserum generated against indicated antigen; RT-PCR - reverse transcriptase polymerase chain reaction; ICKA - immune-complex kinase assay; ITAM - immunoreceptor tyrosine-based activation motifs; SH2 and SH3 - Src homology domains 2 and 3; PBS - phosphate-buffered saline.
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