Words similar to icka
Example sentences for: icka
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BCR-induced interaction between Cbl and Syk increases the susceptibility of Syk to proteolysis [ 55 ] , possibly through enhancement of ubiquitination [ 56 ] . Expression of a dominant-negative SHP-1 phosphatase in the mature murine B cell line K46 resulted in an increase in BCR-induced Syk kinase activity as measured by C3b phosphorylation in an ICKA and enhanced BCR-induced Ca 2+fluxes [ 57 ] . It remains to be determined if interference with any of these known regulatory proteins is responsible for the dominant-positive effect.
Immunoprecipitates were generated from 2 × 10 7cells as described for the ICKA except that the immunoprecipitates were washed once with PBS and resuspended in SDS Sample Buffer.
Second, molecules with the Y130E mutations did not co-immunoprecipitate with the BCR [ 58 ] . A second example involves an in vitro model of histamine release in the Syk-deficient RBL-2H3 cell line [ 59 ] . When tyrosines 519 and 520 were mutated and introduced into Syk-deficient RBL-2H3 cells, these Syk molecules were capable of phosphorylation of c3b substrate in an ICKA.
To examine the ability of Syk from cells that expressed (K395A/3F)Syk to phosphorylate an exogenous Syk target, c3b-GST was added to the Syk ICKA (Figure 4, lower panels).
In unstimulated clones expressing the (K395A/3F)Syk molecule, the possibility existed that the intense band observed in the ICKA (Figure 3Dlanes 4, 7, and 10) was not due to increased Syk activity, but rather to the ability of the background levels of active endogenous Syk to preferentially phosphorylate the (K395A/3F)Syk molecule due to possible changes in its three-dimensional structure.
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