Words similar to icka

Example sentences for: icka

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Immune complex kinase assay (ICKA)
Immunoprecipitates were generated from 2 × 10 7cells as described for the ICKA except that the immunoprecipitates were washed once with PBS and resuspended in SDS Sample Buffer.
To examine the ability of Syk from cells that expressed (K395A/3F)Syk to phosphorylate an exogenous Syk target, c3b-GST was added to the Syk ICKA (Figure 4, lower panels).
IgM - immunoglobulin of the M isotype; ova - chicken ovalbumin; PY - phosphotyrosine; PLCγ2 - phospholipase C γ2; anti - antibody or antiserum generated against indicated antigen; RT-PCR - reverse transcriptase polymerase chain reaction; ICKA - immune-complex kinase assay; ITAM - immunoreceptor tyrosine-based activation motifs; SH2 and SH3 - Src homology domains 2 and 3; PBS - phosphate-buffered saline.
BCR-induced interaction between Cbl and Syk increases the susceptibility of Syk to proteolysis [ 55 ] , possibly through enhancement of ubiquitination [ 56 ] . Expression of a dominant-negative SHP-1 phosphatase in the mature murine B cell line K46 resulted in an increase in BCR-induced Syk kinase activity as measured by C3b phosphorylation in an ICKA and enhanced BCR-induced Ca 2+fluxes [ 57 ] . It remains to be determined if interference with any of these known regulatory proteins is responsible for the dominant-positive effect.