Words similar to ibat

Example sentences for: ibat

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In addition, IBAT may be yet another apical membrane transport protein that is regulated by CFTR.
Polyclonal rabbit antibodies were obtained from a commercial source (Research Genetics, Huntsville, AL) against a C-terminal 14 amino acid oligopeptide fragment of IBAT and purified by immuno-affinity chromatography as described [ 17 ] . The ileal tissue segments were snap-frozen in -70°C cold isopentane and then stored in -70°C until the tissues were processed.
Prior to use for Western blotting, brush border membrane vesicle suspensions were tested for enrichment of brush border enzymes and showed a 4 - 5-fold increase of alkaline phosphatase and a 10 - 11-fold increase in alpha-glucosidase activity using standard methods [ 19 ] . On densitometric evaluations of Western blots, IBAT concentrations were twice as high in heterozygotes as in controls (17 ± 3 vs. 5 ± 1 density units; p < 0.05) and four times as high in homozygotes (30 ± 4 vs. 5 ± 1 density units; p < 0.05) (Fig.
Blots were developed using a 1:100 dilution of the rabbit anti-hamster IBAT antibodies and an amplified alkaline phosphatase goat-anti-rabbit immunoblot assay kit (Biorad, Hercules, CA) as recommended by the manufacturer.
A 1:20 dilution of anti-IBAT protein antibodies was applied for one hour.