Words similar to hypotonic
Example sentences for: hypotonic
How can you use “hypotonic” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cells were lysed with hypotonic lysis buffer with protease inhibitors (Roche/Boehringer Mannheim, Indianapolis, USA).
Similar to previously described protocols [ 21 22 30 ] , cell lysates were prepared by hypotonic lysis of untreated LNCaP cells or cells treated with MIB for 96 h.
At two months of age, she was mildly hypotonic on a follow up evaluation and the diagnosis of a possible mitochondrial cytopathy was suspected.
The cell pellet was washed once in cold phosphate-buffered saline and subsequently resuspended in hypotonic lysis buffer (10 mM Tris.Cl, pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 1 mM phenylmethylsulfonyl fluoride, 2 μg/ml pepstatin, 2 μg/ml leupeptin, 5 μg/ml aprotinin, 1 mM dithiothreitol).
The cells were transferred to microfuge tubes, pelleted (5 seconds at 14,000 rpm, Eppendorf 5415C, 4°C) and resuspended in hypotonic Buffer A (10 mM K-HEPES pH 7.9, 1.5 mM MgCl 2 , and 10 mM KCl, with inhibitors added as for the media above) for 15 minutes on ice.