Words similar to hypotonic
Example sentences for: hypotonic
How can you use “hypotonic” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cells were lysed with hypotonic lysis buffer with protease inhibitors (Roche/Boehringer Mannheim, Indianapolis, USA).
Cell lysates for ubiquitylation assays were prepared by hypotonic lysis on ice in 20 mM Hepes pH 7.5, 15 mM MgCl 2 , 5 mM KCl, 1 mM DTT, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 1 mM PMSF, 17 μg/ml aprotinin as described previously [ 30 ] . Lysates were cleared by centrifugation and used without prior freezing.
Splenocytes were mechanically dissociated by disruption through steel mesh, and red blood cells were lysed using a hypotonic ammonium chloride solution (82.
Cells were washed with phosphate-buffered saline and then lysed in 0.5 ml hypotonic lysis buffer by 10 passages through a 27-gauge needle.
Similar to previously described protocols [ 21 22 30 ] , cell lysates were prepared by hypotonic lysis of untreated LNCaP cells or cells treated with MIB for 96 h.
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