Example sentences for: hypotonic

How can you use “hypotonic” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cells were lysed with hypotonic lysis buffer with protease inhibitors (Roche/Boehringer Mannheim, Indianapolis, USA).

  • Similar to previously described protocols [ 21 22 30 ] , cell lysates were prepared by hypotonic lysis of untreated LNCaP cells or cells treated with MIB for 96 h.

  • At two months of age, she was mildly hypotonic on a follow up evaluation and the diagnosis of a possible mitochondrial cytopathy was suspected.

  • The cell pellet was washed once in cold phosphate-buffered saline and subsequently resuspended in hypotonic lysis buffer (10 mM Tris.Cl, pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 1 mM phenylmethylsulfonyl fluoride, 2 μg/ml pepstatin, 2 μg/ml leupeptin, 5 μg/ml aprotinin, 1 mM dithiothreitol).

  • The cells were transferred to microfuge tubes, pelleted (5 seconds at 14,000 rpm, Eppendorf 5415C, 4°C) and resuspended in hypotonic Buffer A (10 mM K-HEPES pH 7.9, 1.5 mM MgCl 2 , and 10 mM KCl, with inhibitors added as for the media above) for 15 minutes on ice.


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