Words similar to hypotonic
Example sentences for: hypotonic
How can you use “hypotonic” in a sentence? Here are some example sentences to help you improve your vocabulary:
Cell lysates for ubiquitylation assays were prepared by hypotonic lysis on ice in 20 mM Hepes pH 7.5, 15 mM MgCl 2 , 5 mM KCl, 1 mM DTT, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 1 mM PMSF, 17 μg/ml aprotinin as described previously [ 30 ] . Lysates were cleared by centrifugation and used without prior freezing.
Tetraethylammonium is known as a pore-occluding blocker of voltage-gated potassium channels [ 1 ] , but it also blocks other cationic channels such as calcium-dependent K +channels [ 2 3 ] and the nicotinic acetylcholine receptor [ 4 ] . TEA at 0.1 to 10 mM also inhibits osmotic water flux through human AQP1 channels expressed in Xenopus oocytes, decreasing the net swelling rate in hypotonic saline by 30-40% as compared to AQP1-expressing oocytes not treated with TEA [ 5 ] . This blocking effect on osmotic water flux was demonstrated to involve AQP1 channels specifically by using site-directed mutagenesis (tyrosine 186 to phenylalanine) to generate a Y186F AQP1 channel that is insensitive to block by TEA, but retains sensitivity to block by mercury.
Cells were washed with phosphate-buffered saline and then lysed in 0.5 ml hypotonic lysis buffer by 10 passages through a 27-gauge needle.
At two months of age, she was mildly hypotonic on a follow up evaluation and the diagnosis of a possible mitochondrial cytopathy was suspected.
The cell pellet was washed once in cold phosphate-buffered saline and subsequently resuspended in hypotonic lysis buffer (10 mM Tris.Cl, pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 1 mM phenylmethylsulfonyl fluoride, 2 μg/ml pepstatin, 2 μg/ml leupeptin, 5 μg/ml aprotinin, 1 mM dithiothreitol).
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