Words similar to hypotonic

Example sentences for: hypotonic

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Cell lysates for ubiquitylation assays were prepared by hypotonic lysis on ice in 20 mM Hepes pH 7.5, 15 mM MgCl 2 , 5 mM KCl, 1 mM DTT, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 1 mM PMSF, 17 μg/ml aprotinin as described previously [ 30 ] . Lysates were cleared by centrifugation and used without prior freezing.
At two months of age, she was mildly hypotonic on a follow up evaluation and the diagnosis of a possible mitochondrial cytopathy was suspected.
Cells were lysed with hypotonic lysis buffer with protease inhibitors (Roche/Boehringer Mannheim, Indianapolis, USA).
The cell pellet was washed once in cold phosphate-buffered saline and subsequently resuspended in hypotonic lysis buffer (10 mM Tris.Cl, pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 1 mM phenylmethylsulfonyl fluoride, 2 μg/ml pepstatin, 2 μg/ml leupeptin, 5 μg/ml aprotinin, 1 mM dithiothreitol).
After lysis of cells in hypotonic buffer followed by high-speed centrifugation, α i2 was found predominantly in the particulate fraction from COS (Figure 2E, lanes 3 and 4) or BHK cells (Figure 2E, lanes 9 and 10).