Example sentences for: hypotonic

How can you use “hypotonic” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Cells were lysed with hypotonic lysis buffer with protease inhibitors (Roche/Boehringer Mannheim, Indianapolis, USA).

  • Cell lysates for ubiquitylation assays were prepared by hypotonic lysis on ice in 20 mM Hepes pH 7.5, 15 mM MgCl 2 , 5 mM KCl, 1 mM DTT, 10 μg/ml leupeptin, 10 μg/ml pepstatin, 1 mM PMSF, 17 μg/ml aprotinin as described previously [ 30 ] . Lysates were cleared by centrifugation and used without prior freezing.

  • Splenocytes were mechanically dissociated by disruption through steel mesh, and red blood cells were lysed using a hypotonic ammonium chloride solution (82.

  • Cells were washed with phosphate-buffered saline and then lysed in 0.5 ml hypotonic lysis buffer by 10 passages through a 27-gauge needle.

  • Similar to previously described protocols [ 21 22 30 ] , cell lysates were prepared by hypotonic lysis of untreated LNCaP cells or cells treated with MIB for 96 h.


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast