Words similar to hydroxyethylpiperazine-
Example sentences for: hydroxyethylpiperazine-
How can you use “hydroxyethylpiperazine-” in a sentence? Here are some example sentences to help you improve your vocabulary:
pR, proteorhodopsin; bR, bacteriorhodopsin; OG, β-octyl-D-glucoside; SDS, sodium dodecylsulfate; PAGE, polyacrylamide gel electrophoresis; pR-TCM, pR triple cysteine mutant [C(107,156,175)S]; DHPC, 1,2-diheptanoyl-SN-glycero-3-phosphocholine; DMPC, dimyristoylphosphatidylcholine; HEPES, N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid
A putative HEPES (N-2 hydroxyethylpiperazine-N'-2-ethanesulphonic acid) molecule is present in the binding pocket of the template structure.
THP-1 (generously provided by Dr P Guyre, Dartmouth Medical School), Jurkat (ATCC), and 293 cell lines were cultured in RPMI complete medium (CM), consisting of RPMI 1640 with 10% heat-inactivated fetal bovine serum, 0.1 ml of sodium pyruvate, 2.0 mmol/l of L-glutamine, 25 mmol/l of 1 M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid), 0.1 mmol/l of nonessential amino acids, 0.25 μg/ml of amphotericin, 50 μg/ml gentamicin (all from BioWhittaker, Walkersville, MD, USA) and 5 × 10 -5M 2-mercaptoethanol (Sigma, St Louis, MO, USA).
At the time of experimental treatment, the culture media was replaced with Locke's buffer containing (in mM) 154 NaCl, 5.6 KCl, 2.3 CaCl 2 , 1 MgCl 2 , 3.6 NaHCO 3 , 5 glucose, 5 N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (pH 7.2) and neurons were incubated with culture supernatants from HIV infected monocytic cells, subtoxic concentrations of Tat (40 nM) + gp120 (30 pM), various concentrations of methamphetamine or cocaine, or viral proteins + methamphetamine and cocaine.
BSA = bovine serum albumin; CF = cystic fibrosis; CFTR = cystic fibrosis transmembrane conductance regulator; C L = lethal concentration; DMSO = dimethyl sulfoxide; EC 50 = effective concentration 50%; EDTA = ethylenediamine tetraacetic acid; GGPG = modified PG-1 with a glycine-rich hexapeptide extension (GGGYGG) containing a single tyrosine residue; HEPES = N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid; HPLC = high performance liquid chromatography; I-GGPG = monoiodinated variant of GGPG; LPS = lipopolysaccharide; MAC = minimal active concentration; PG-1 = protegrin-1; RU = resonance units; SDS = sodium dodecyl sulfate; SPR = surface plasmon resonance; TSB = trypticase soy broth.
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