Words similar to hydrolyzed
Example sentences for: hydrolyzed
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β-Galactosidase activity was determined as previously described [ 4 ] . The protein concentration was estimated by assuming that a cell density at OD 600 of 1.4 corresponds to 150 μg protein per ml [ 32 ] . β-galactosidase activity is expressed in nanomol ONPG hydrolyzed per min per mg protein.
Although the naturally occurring forms of vitamin E have lipid-soluble antioxidant properties that protect cell membranes against damage by free radicals, the acetate and succinate derivatives that are esterified at the C-6 position of the chromanol ring do not have antioxidant properties unless the esterification is hydrolyzed and free tocopherol is regenerated [ 1 2 3 ] .
predicted that Phe158 and Ile 164 form hydrophobic interactions with bulky substituents on the substrate, suggesting that the loss of these residues would only affect binding of substrates with large aromatic substituents [ 37 ] . In the modeling studies on CcrA [ 42 ] , Asn233 was predicted to interact with the β-lactam carbonyl on substrate, and mutagenesis studies have supported this prediction [ 43 ] . Although Asn233 is sequence conserved in L1 [ 35 ] , it is located 14Å away from the modeled position of the β-lactam carbonyl and was predicted not to play a role in substrate binding to L1 [ 37 ] . On the other hand, the substrate-binding model predicted that Tyr228 was in position to offer a hydrogen bond to the β-lactam carbonyl and participate in an oxyanion hole that was proposed to form as the substrate was hydrolyzed [ 37 ] . By using the crystal structure and modeling studies on L1, Ullah et al.
The reaction was carried out at 42°C for 120 min and the residual RNA was hydrolyzed with 0.1 mM EDTA and 0.2 M NaOH at 65°C for 15 min.
The cap is formed by three enzymatic reactions: the 5' triphosphate end of the nascent pre-mRNA is hydrolyzed to a diphosphate by RNA triphosphatase; the diphosphate end is capped with GMP by RNA guanylyltransferase; and the GpppN cap is methylated by RNA (guanine-7-) methyltransferase [ 1 ] .
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