Words similar to hydrolyzed
Example sentences for: hydrolyzed
How can you use “hydrolyzed” in a sentence? Here are some example sentences to help you improve your vocabulary:
in situ RNA hybridization on BrDU-labeled embryos was carried out as described previously [ 59] except that the probes were not hydrolyzed.
RT was halted with 10 μL 0.5 M EDTA, and RNA hydrolyzed by the addition of 10 μL 1 N NaOH and incubation at 65°C for 15 minutes.
The cap is formed by three enzymatic reactions: the 5' triphosphate end of the nascent pre-mRNA is hydrolyzed to a diphosphate by RNA triphosphatase; the diphosphate end is capped with GMP by RNA guanylyltransferase; and the GpppN cap is methylated by RNA (guanine-7-) methyltransferase [ 1 ] .
The reaction was carried out at 42°C for 120 min and the residual RNA was hydrolyzed with 0.1 mM EDTA and 0.2 M NaOH at 65°C for 15 min.
β-Galactosidase activity was determined as previously described [ 4 ] . The protein concentration was estimated by assuming that a cell density at OD 600 of 1.4 corresponds to 150 μg protein per ml [ 32 ] . β-galactosidase activity is expressed in nanomol ONPG hydrolyzed per min per mg protein.