Words similar to hydrolyzed
Example sentences for: hydrolyzed
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Soil samples of 5 to 15 g were used for supercritical fluid extraction (SFE) with supercritical CO 2 (SCCO 2 ). Samples were first hydrolyzed in 5 to 10 ml of acetone containing 2 M NH 4 OH under ultrasound agitation (water bath at 160 watts) for 1 h at room temperature.
Nonetheless, bacterial resistance to these antibiotics is extensive, most commonly due to the bacterial production of β-lactamases [ 10 51 ] . In fact, there have been over 300 distinct β-lactamases reported, and most of these enzymes utilize an active site serine group to nucleophilically attack the β-lactam carbonyl, resulting in a hydrolyzed product that is covalently attached to the active site.
The structure of the bovine mitochondrial F 1 -ATPase has been determined by X-ray crystallography [ 30 ] . The intein insertion points in the yeasts' V-ATPase and the Thermoplasma and Pyrococcus A-ATPases correspond to the catalytic site where the ATP binds and is hydrolyzed during the catalytic cycle.
Reactions were quenched by the addition of 10 μl of 0.5 M EDTA and RNA template was hydrolyzed by the addition of 10 μl of 1 M NaOH followed by heating at 70°C for 10 min.
The inability to propose a consistent binding model also supports the recent proposal that different substrates of L1 are hydrolyzed by different mechanisms and further suggests that using steady-state kinetic constants may not be a valid way to probe substrate binding to L1.
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