Words similar to hydrolyzed
Example sentences for: hydrolyzed
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predicted that Phe158 and Ile 164 form hydrophobic interactions with bulky substituents on the substrate, suggesting that the loss of these residues would only affect binding of substrates with large aromatic substituents [ 37 ] . In the modeling studies on CcrA [ 42 ] , Asn233 was predicted to interact with the β-lactam carbonyl on substrate, and mutagenesis studies have supported this prediction [ 43 ] . Although Asn233 is sequence conserved in L1 [ 35 ] , it is located 14Å away from the modeled position of the β-lactam carbonyl and was predicted not to play a role in substrate binding to L1 [ 37 ] . On the other hand, the substrate-binding model predicted that Tyr228 was in position to offer a hydrogen bond to the β-lactam carbonyl and participate in an oxyanion hole that was proposed to form as the substrate was hydrolyzed [ 37 ] . By using the crystal structure and modeling studies on L1, Ullah et al.
RT was halted with 10 μL 0.5 M EDTA, and RNA hydrolyzed by the addition of 10 μL 1 N NaOH and incubation at 65°C for 15 minutes.
The structure of the bovine mitochondrial F 1 -ATPase has been determined by X-ray crystallography [ 30 ] . The intein insertion points in the yeasts' V-ATPase and the Thermoplasma and Pyrococcus A-ATPases correspond to the catalytic site where the ATP binds and is hydrolyzed during the catalytic cycle.
Soil samples of 5 to 15 g were used for supercritical fluid extraction (SFE) with supercritical CO 2 (SCCO 2 ). Samples were first hydrolyzed in 5 to 10 ml of acetone containing 2 M NH 4 OH under ultrasound agitation (water bath at 160 watts) for 1 h at room temperature.
The cap is formed by three enzymatic reactions: the 5' triphosphate end of the nascent pre-mRNA is hydrolyzed to a diphosphate by RNA triphosphatase; the diphosphate end is capped with GMP by RNA guanylyltransferase; and the GpppN cap is methylated by RNA (guanine-7-) methyltransferase [ 1 ] .
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