Words similar to hydrolyzed
Example sentences for: hydrolyzed
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The input RNA was hydrolyzed by adding 15 μl 0.1 M NaOH/2 mM EDTA and incubated at 65°C for 8 min, followed by neutralization with 15 μl 0.1 M HCl.
Reactions were quenched by the addition of 10 μl of 0.5 M EDTA and RNA template was hydrolyzed by the addition of 10 μl of 1 M NaOH followed by heating at 70°C for 10 min.
predicted that Phe158 and Ile 164 form hydrophobic interactions with bulky substituents on the substrate, suggesting that the loss of these residues would only affect binding of substrates with large aromatic substituents [ 37 ] . In the modeling studies on CcrA [ 42 ] , Asn233 was predicted to interact with the β-lactam carbonyl on substrate, and mutagenesis studies have supported this prediction [ 43 ] . Although Asn233 is sequence conserved in L1 [ 35 ] , it is located 14Å away from the modeled position of the β-lactam carbonyl and was predicted not to play a role in substrate binding to L1 [ 37 ] . On the other hand, the substrate-binding model predicted that Tyr228 was in position to offer a hydrogen bond to the β-lactam carbonyl and participate in an oxyanion hole that was proposed to form as the substrate was hydrolyzed [ 37 ] . By using the crystal structure and modeling studies on L1, Ullah et al.
The inability to propose a consistent binding model also supports the recent proposal that different substrates of L1 are hydrolyzed by different mechanisms and further suggests that using steady-state kinetic constants may not be a valid way to probe substrate binding to L1.
RNA was hydrolyzed with 200 mM NaOH and 100 mM EDTA for 15 min at 65°C, then neutralized with 200 mM HCl.
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