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Example sentences for: huvecs

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Model system employed in our study closely resembles with those that have been described previously [ 7 8 9 ] . Either human microvascular endothelial cells (HMECs) or human umbilical vein endothelial cells (HUVECs) were seeded between two thin layers of type I collagen gels, and overlayed with complete media supplemented with VEGF 165, as described in methods.
HUVECs were purchased from Clonetics (CA).
HUVECs were cultured in a 3D collagen matrix for 0, 12, 24, and 36 hours, with or without VEGF 165, as described above.
HUVECs were cultured in a 3D collagen matrix for 0, 12, 24, 36, 48, and 72 hours, with or without VEGF 165and total RNA was extracted with TRIzol ®Reagent (Invitrogen Corp).
Monolayer cell culture was performed as previously described [ 46 47 ] . To induce capillary formation, unstarved proliferating HUVECs (passage 4) were gently resuspended (at 6 × 10 5cells/ml) in a 3D collagen matrix that was prepared by mixing 7 ml of 3.0 mg/ml type I collagen solution with 1 ml of 10X M199 medium at 4°C, adjusting the pH to 7.5 with 0.1 N sodium hydroxide, adding 0.1 ml of 100X ITS, and the final volume to 10 ml with sterile distilled water.