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Example sentences for: huvecs

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HUVECs were cultured in a 3D collagen matrix for 0, 12, 24, 36, 48, and 72 hours, with or without VEGF 165and total RNA was extracted with TRIzol ®Reagent (Invitrogen Corp).
Monolayer cell culture was performed as previously described [ 46 47 ] . To induce capillary formation, unstarved proliferating HUVECs (passage 4) were gently resuspended (at 6 × 10 5cells/ml) in a 3D collagen matrix that was prepared by mixing 7 ml of 3.0 mg/ml type I collagen solution with 1 ml of 10X M199 medium at 4°C, adjusting the pH to 7.5 with 0.1 N sodium hydroxide, adding 0.1 ml of 100X ITS, and the final volume to 10 ml with sterile distilled water.
HUVECs were cultured in a 3D collagen matrix for 0, 12, 24, and 36 hours, with or without VEGF 165, as described above.
Model system employed in our study closely resembles with those that have been described previously [ 7 8 9 ] . Either human microvascular endothelial cells (HMECs) or human umbilical vein endothelial cells (HUVECs) were seeded between two thin layers of type I collagen gels, and overlayed with complete media supplemented with VEGF 165, as described in methods.
HUVECs were purchased from Clonetics (CA).