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Example sentences for: huvec

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Confluent HUVEC were washed with HBSS and incubated for 30 min at 37°C with various agonists in HBSS with 0.35%BSA: HBSS alone; 0.5 % dimethyl sulfoxide (DMSO); ionophore A23187 1-100 μmol/L; phorbol myristate acetate (PMA) 1 μg/mL; human thrombin receptor activating peptide SFLLRNP (TRAP) 1-50 μmol/L; human thrombin (THR) US FDA standard lot J (Bethesda, MD) 0.1-10 NIH Unit/mL; human tissue plasminogen activator (TPA) 3.5 kU/mL or human angiotensin II (AGTII) 1 μmol/L.
HUVEC cultures in 6-well plates were photographed using CK40 Olympus culture microscope (Olympus, Japan) with a SPOT camera and software (Diagnostic Instruments, Inc., MI, USA).
Our previous study demonstrated that cultured HUVEC released annexin V-binding membrane MP in response to proapoptotic stimulation with CPT [ 3 ] . In the present study, we investigated whether endothelial cells release membrane MP after rapid stimulation with Ca 2+-mobilizing agents or after overnight stimulation with proinflammatory agents TNFα and LPS, or only after induction of apoptosis.
6) suggests that HUVEC can release different types of annexin V-binding MP with respect to their antigen expression.
ZVAD also inhibited a release of MP from unstimulated cells, which probably represents spontaneous apoptosis of cultured HUVEC (Fig.