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Example sentences for: huvec

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Supernatants obtained from PBMC exposed to IL-2 or OPTI-MEM (control) for 3 and 24 h were overlayed on confluent monolayers of HUVEC in T175 flasks (Falcon) at 37°C in 5% CO 2 for 3 h.
Treatment of the enriched PBMC with IL-2 (6,000 IU/ml) increased HUVEC permeability (compared to PBMC alone) only after 24 hours (Student t -test p = 0.02).
These discrepant results may stem from differences in HUVEC culture conditions and the flow cytometric assay.
Confluent HUVEC were washed with HBSS and incubated for 30 min at 37°C with various agonists in HBSS with 0.35%BSA: HBSS alone; 0.5 % dimethyl sulfoxide (DMSO); ionophore A23187 1-100 μmol/L; phorbol myristate acetate (PMA) 1 μg/mL; human thrombin receptor activating peptide SFLLRNP (TRAP) 1-50 μmol/L; human thrombin (THR) US FDA standard lot J (Bethesda, MD) 0.1-10 NIH Unit/mL; human tissue plasminogen activator (TPA) 3.5 kU/mL or human angiotensin II (AGTII) 1 μmol/L.
ZVAD also inhibited a release of MP from unstimulated cells, which probably represents spontaneous apoptosis of cultured HUVEC (Fig.