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Example sentences for: huvec

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In a study of MP-release in subconfluent, confluent and overgrown HUVEC culture, the cells at 24 hrs (50% of confluence), 48 hrs (reaching confluence) or 72 hrs (overgrown) in culture were incubated for 24 hrs with 0.5 % DMSO, 500 nM or 5 μM CPT in medium.
The only report showing MP-release after rapid stimulation with a physiologic agonist is the study by Hamilton et al . [ 21 ] that demonstrated MP-release from HUVEC with the complement complex C5b-9.
Confluent HUVEC were washed with HBSS and incubated for 30 min at 37°C with various agonists in HBSS with 0.35%BSA: HBSS alone; 0.5 % dimethyl sulfoxide (DMSO); ionophore A23187 1-100 μmol/L; phorbol myristate acetate (PMA) 1 μg/mL; human thrombin receptor activating peptide SFLLRNP (TRAP) 1-50 μmol/L; human thrombin (THR) US FDA standard lot J (Bethesda, MD) 0.1-10 NIH Unit/mL; human tissue plasminogen activator (TPA) 3.5 kU/mL or human angiotensin II (AGTII) 1 μmol/L.
IL-2 alone had minimal effects on HUVEC.
HUVEC were obtained from Clonetics (San Diego, CA) and cultured in endothelial cell growth media EGM-2 (Clonetics) containing 2% of fetal bovine serum and supplements.