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Example sentences for: huvec
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The essential step in plasma membrane destabilization and MP-release very likely is a caspase-dependent sphingomyelin hydrolysis, which leads to the production of ceramide [ 28 29 30 ] . It is possible, however, that sphingomyelin hydrolysis and MP-release may be a consequence of the action of initiator caspases or other ZVAD sensitive proteases rather than only executioner caspases [ 15 31 32 ] . Our study was performed in HUVEC culture, which may have particular properties in respect of MP release when compared with endothelial cells from other organs.
Phase contrast microscopy showed that HUVEC culture treated for 24 hrs with 5 μM CPT (Fig.
AGTII, angiotensin II; BSA, bovine serum albumin; CHX, cycloheximide; CPT, camptothecin; DMSO, dimethyl sulfoxide; FITC, fluorescein isothiocyanate; HBSS, Hanks' balanced salt solution; HUVEC, human umbilical vein endothelial cells; IC-IgG, IgG isotype control; Mab, monoclonal antibody; MP, microparticles; PE, phycoerythrin; PI, propidium iodide; PMA, phorbol-myristate-acetate; PS, phosphatidylserine; TRAP, thrombin receptor activating peptide (SFLLRNP); TNF, tumor necrosis factor; TPA, tissue plasminogen activator; TUNEL, terminal deoxynucleotidyltransferase dUTP nick end labeling; ZVAD, Z-Val-Ala-Asp-fluoromethyl ketone.
Comparison of FSC values of standard beads and analyzed MP showed that 95% of annexin V-binding MP released from HUVEC were < 1 μm in size (range 0.3 - 3.0 μm).
HUVEC: Human Umbilical Vein Endothelial Cell