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Example sentences for: hspgs

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In addition, the presence of heparin in the lysate inhibited the 46 kDa and 70 kDa HSPGs from binding to the III1-C column (Fig.
RASMC HSPGs were analyzed further by immunoblotting with the monoclonal antibody 3G10 which specifically recognizes desaturated uronates on the heparan sulfate stubs that remain associated with core proteins after heparitinase digestion [ 37 ] . Complete digestion of RASMC lysates with heparitinase reveals major bands at 46 kDa and 70 kDa, indicating that these are the sizes of the major HSPG core proteins (not shown).
These results indicate that most of the cell surface HSPGs of RASMCs can bind to III1-C in affinity chromatography.
The particular HSPGs that mediate ERK1/2 activation in Jurkat T cells have not been identified, however, the authors speculate that syndecans may participate in the adhesion and signaling in that system [ 38 ] . This may also be the case in our system, as described below.
Heparitinase and chondroitinase ABC treatment of the 35SO 4 labeled samples revealed that the 450 kDa proteoglycan was composed mostly of HSPGs (Fig.