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Example sentences for: hsa
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To minimize UCB oxidation, all tubes and solutions were deoxygenated with Argon and kept in the dark [ 31 ] . To determine the [UCB] in the UCB/DMSO stock, 6.0 μL, was added to 3.0 mL of the HSA stock.
Primarily examined through the use of G-banding cytogenetic techniques, the human and common chimpanzee karyotypes differ by only 10 euchromatic rearrangements: a telomere fusion between PTR chromosomes 12 and 13, resulting in HSA chromosome 2, and 9 pericentric inversions (HSA 1, 4, 5, 9, 12, 15, 16, 17, 18) [ 1 2 ] . The predominance of pericentric inversions between chimps and humans highlights their potential importance in the divergence of human from non-human primate species, and provides an opportunity to investigate the mechanism facilitating these rearrangements.
We then defined the inversion breakpoint interval as the region between the most distal probe on HSA 15q to display a PTR XVp signal, RP11-360J18 and the most proximal probe on HSA 15q to display a PTR XVq signal, RP11-736I24 (Figure 3).
The absorbance, A , at 460 nm was read against a blank containing 2.0 mL of HSA stock plus 4.0 μL DMSO, and the [UCB] calculated using the extinction coefficient, ε, 47,000 M -1.cm -1 [ 18 ] . The pH measurement of each final DMSO/aqueous buffer system included an electrode calibration using the strong acid, HClO 4 [ 8 ] . The 0.1 M phosphate buffer in N = 0.31 DMSO was centrifuged because of partial insolubility [ 8 ] and only the supernatant was used.
The karyotypes of humans and the African and Asian great apes are remarkably well conserved, with relatively few large-scale chromosomal changes among these species despite the considerable phenotypic and biological differences between hominoids [ 1 2 3 ] . This conservation is particularly relevant in comparisons between the human ( Homo sapiens ; HSA) and common chimpanzee ( Pan troglodytes ; PTR) genomes, for in order to completely ascertain the evolution of our own lineage it is necessary to understand what differentiates us at the genomic level from our closest relatives.
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