Words similar to hpaii

Example sentences for: hpaii

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Genomic DNA (approximately 200 ng) was digested overnight using a methylation-sensitive restriction enzyme HpaII, as per the manufacturer's instructions (Gibco BRL, Rockville, MD, USA).
The PCR products of each sample (mock and HpaII) were diluted 1:10 in the same vial.
Clonality was further confirmed and X chromosome inactivation status was determined by the length of the polymorphic CAG trinucleotide repeat on the inactive androgen receptor allele [ 21 22 ] . Restriction digestion of four restriction sites between the flanking primers and adjacent to the trinucleotide repeat were carried out with the methylation sensitive enzymes HpaII and HhaI.
A mock sample as control was prepared simultaneously without the HpaII enzyme.
These primers flank the triple repeat CAG and the HpaII sites in the first exon of the AR gene (Fig.