Words similar to hpa
Example sentences for: hpa
How can you use “hpa” in a sentence? Here are some example sentences to help you improve your vocabulary:
The pKK-RT66 clone containing two unique restriction sites, Hpa1 and Stu1 , at codons 136 and 140 in the RT coding region [ 15 ] was used for insertion of 6 amino-acid residues in the β7-β8 loop of the p66 and p51 subunit.
The positive clones were screened in E. coli HB101 by the absence of an Hpa1 site and the correct orientation of the insertion was confirmed by DNA sequencing.
pJC14 was a Hpa I- Bsp EI deletion (309 bp) of pJC10, and pJC15 was a Bgl II- Bsp EI deletion (150 bp) of pJC10.
By performing PCR amplification across this region after cutting with the methylation-sensitive enzyme Hpa II, the relative amounts of the methylated AR alleles can be quantitatively determined with a high degree of accuracy; variance on repeated assays is approximately 4% [ 12].
1. 5) The M. tuberculosis sigE gene was PCR amplified from H37Rv genomic DNA using primer 987917 and primer 987916 (which contains a Bam HI site), digested with Bam HI, and cloned into pMV261 digested with Hpa I and Bam HI.