Words similar to hpa

Example sentences for: hpa

How can you use “hpa” in a sentence? Here are some example sentences to help you improve your vocabulary:

The 18-mer duplex DNA encoding the insertion peptide (Ile-Asn-Asn-Glu-Thr-Ile) was ligated with Hpa1 digested pKK-RT66 in between codon 135 and 136.
pΔHprt was constructed by introducing a 2.5 Kb BamHI/HindIII fragment of 5' hprt genomic DNA (E14TG2a origin) from the plasmid pMP8 into the Hpa1 site of pOS.DUP/DEL [ 15 ] . The ends of the fragments were made blunt using Klenow.
1. 2) A 760-bp fragment containing 526 bp of the 5' region of the M. smegmatis sigE gene and 234 bp of the region upstream was PCR amplified using primer 7206 and primer 7207 (which contains a Hpa I restriction site), digested with Hpa I, and cloned into the Hpa I site of pSEKO.
pJC16 arrays induced fewer migration defects than pJC14 arrays and pJC17 arrays induced fewer migration defects than pJC16 arrays, so there appeared to be multiple sequences between the Hpa I and Bsa BI restriction sites that contributed to the migration defect.
1) A 757-bp fragment containing 246 bp of the 3' end of the M. smegmatis sigE gene and 511 bp of the 5' end of the adjacent ORF1 gene was PCR amplified using primer 7208 (which contains a Hpa I restriction site) and primer 7209 and cloned into the Eco RV blunt-cloning site of pT7Blue to generate pSEKO.