Words similar to hpa
Example sentences for: hpa
How can you use “hpa” in a sentence? Here are some example sentences to help you improve your vocabulary:
3) The gentamicin-resistance gene ( aacC1 ) was PCR-amplified from pPR27 as a 1270-bp fragment using primer 7204 and primer 7205 (both of which contain Hpa I restriction sites), digested with Hpa I, and cloned into the Hpa I site located between the two fragments of the sigE gene in pSEKO.
By performing PCR amplification across this region after cutting with the methylation-sensitive enzyme Hpa II, the relative amounts of the methylated AR alleles can be quantitatively determined with a high degree of accuracy; variance on repeated assays is approximately 4% [ 12].
Hpa II/polymerase chain reaction assay for X-chromosome inactivation
Recent evidence indicates that the cytokine, leukemia inhibitory factor (LIF) and its functional binding to the high affinity receptor, a heterodimer between low-affinity LIFR and glycoprotein 130 (LIFR:gp130), are players within the HPA cascade of events.
Employing a modification of previously described methods [ 11], we utilized a fluorescent Hpa II/PCR assay of the AR locus to assess X-chromosome inactivation patterns.