Example sentences for: hpa

How can you use “hpa” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The pKK-RT66 clone containing two unique restriction sites, Hpa1 and Stu1 , at codons 136 and 140 in the RT coding region [ 15 ] was used for insertion of 6 amino-acid residues in the β7-β8 loop of the p66 and p51 subunit.

  • The positive clones were screened in E. coli HB101 by the absence of an Hpa1 site and the correct orientation of the insertion was confirmed by DNA sequencing.

  • pJC14 was a Hpa I- Bsp EI deletion (309 bp) of pJC10, and pJC15 was a Bgl II- Bsp EI deletion (150 bp) of pJC10.

  • By performing PCR amplification across this region after cutting with the methylation-sensitive enzyme Hpa II, the relative amounts of the methylated AR alleles can be quantitatively determined with a high degree of accuracy; variance on repeated assays is approximately 4% [ 12].

  • 1. 5) The M. tuberculosis sigE gene was PCR amplified from H37Rv genomic DNA using primer 987917 and primer 987916 (which contains a Bam HI site), digested with Bam HI, and cloned into pMV261 digested with Hpa I and Bam HI.


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