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Example sentences for: hpb-all

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Given the morphologic similarity between neurite outgrowth of N1E-115 cells and the aberrant pseudopodial extension of HPB-ALL when RhoA is inhibited with C3 exoenzyme, it will be interesting to determine if other GTPases such as Rac or Cdc42 are activated to promote this phenotype after C3 treatment of HPB-ALL cells.
After C3 electroporation, HPB-ALL were allowed to spread on VCAM-1 for 1 hour, and TRITC-phalloidin staining demonstrated the complete absence of fine filopodial structures.
Although Rac1 and Cdc42 were identified in western blots of whole cell lysates prepared from the T cell lines HPB-ALL, CEM, HSB (data not shown), and PB human T lymphocytes (data not shown), there was no detection of ribosylation in the lower molecular weight Rac and Cdc42 related GTPases in HPB-ALL cells, indicating the specificity of C3.
By this method, any further ADP ribosylation of Rho can be identified in vitro after in vivo ribosylation, thus serving as a measure of the efficiency of the electroporation procedure for introducing C3 exoenzyme into HPB-ALL cells [ 31 ] . As figure 1Bdemonstrates, electroporation of C3 transferase (Figure 1B, lane 3) prior to cell disruption prevented further ribosylation of Rho proteins in the cell lysates.
Figure 6demonstrates HPB-ALL spreading on VCAM-1 over a period of 1 hour, where images from the first 10 minutes were obtained from recorded videotape, followed by a 60 minute time point.