Words similar to hpb-all

Example sentences for: hpb-all

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By this method, any further ADP ribosylation of Rho can be identified in vitro after in vivo ribosylation, thus serving as a measure of the efficiency of the electroporation procedure for introducing C3 exoenzyme into HPB-ALL cells [ 31 ] . As figure 1Bdemonstrates, electroporation of C3 transferase (Figure 1B, lane 3) prior to cell disruption prevented further ribosylation of Rho proteins in the cell lysates.
Although Rac1 and Cdc42 were identified in western blots of whole cell lysates prepared from the T cell lines HPB-ALL, CEM, HSB (data not shown), and PB human T lymphocytes (data not shown), there was no detection of ribosylation in the lower molecular weight Rac and Cdc42 related GTPases in HPB-ALL cells, indicating the specificity of C3.
Inactivation of Rho by electroporation of C3 exoenzyme did not seem to cause a large overall redistribution of RhoA in spread HPB-ALL.
Electroporation of C3 into HPB-ALL cells
As figure 4demonstrates, untreated HPB-ALL cells display a diffuse staining pattern of RhoA, with small punctate microclusters throughout the normal pseudopodial extensions (figure 4, left panels, arrows).