Words similar to hpb-all
Example sentences for: hpb-all
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When HPB-ALL cells are plated onto β1 integrin substrate such as fibronectin, vascular cell adhesion molecule-1 (VCAM-1), or even antibodies to α4β1 integrins, they respond by extending pseudopodia and displaying a highly flattened, spread morphology (figure 3, panels 4, 7, and 10, and ref [ 32 ] ). However, electroporation of C3 appeared to alter HPB-ALL morphology resulting in the extension of very fine branched finger-like cellular processes when cells were plated on the β1 integrin substrates Fn (figure 3, panel 6), VCAM-1 (figure 3, panel 9), or a mAb specific for β1 integrins (figure 3, panel 12).
To localize filamentous actin within HPB-ALL, cells adherent to VCAM-1 were fixed and stained with TRITC-phalloidin.
After C3 electroporation, HPB-ALL were allowed to spread on VCAM-1 for 1 hour, and TRITC-phalloidin staining demonstrated the complete absence of fine filopodial structures.
Immunolocalization of the GTPase RhoA was performed to determine if inactivation by C3 exoenzyme caused spatial redistribution of RhoA in HPB-ALL.
RhoA immunolocalization in normal and C3 treated HPB-ALL cells spreading on fibronectin
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