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Example sentences for: hotstartaq

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For each amplicon, 1μl of cDNA, 1.5μl of 10 × PCR buffer (Qiagen), 250μM of each dNTP, 0.5 units of HotstarTaq polymerase (Qiagen) and 0.5μM of primer (Life Technologies) were used in a 15μl reaction.
1.5μl of 10 × PCR buffer (Qiagen), 250μM of each dNTP, 0.5 Units of HotstarTaq polymerase (Qiagen) and 0.5μM of primer (Ambion) were used in a 15μl reaction.
Human genomic DNA was amplified by polymerase chain reaction (PCR) usinq Taq polymerase (HotStarTaq, Qiagen) and oligonucleotide primers based on sequences corresponding to the extreme amino- and carboxy-terminal parts of OR ORFs.
For both EGFR and KRAS, PCR was performed using the HotStarTaq Master Mix Kit (Qiagen, Valencia, California, United States), as per manufacturer's instructions.