Words similar to hh

Example sentences for: hh

How can you use “hh” in a sentence? Here are some example sentences to help you improve your vocabulary:

Consistent with its role as an essential pathway inhibitor, removal of Ptc renders the Hh pathway constitutively 'on', independent of the Hh ligand.
Figure 5bshows an immunoblot (anti-HA) of total extracts from HA-Smo-expressing cells treated for 2, 5, 8 and 20 hours with vehicle, 35 nM Hh protein or 0.5 μM Hh agonist.
Having established an in vitro binding assay for the Hh agonist to Smo, we next tested whether the Ptc-independent Hh antagonists could selectively compete with the interaction.
A recent study in Drosophila suggested that Hh stimulates the pathway via Ptc degradation and, as a result, Smo is stabilized through extensive phosphorylation at the plasma membrane where it initiates signaling [ 5 ] . This led to speculation that a Ptc-regulated phosphatase may control the subcellular distribution and stability of Smo [ 5 ] . Although suggestive, the studies in Drosophila did not establish that phosphorylation, translocation or stabilization of Smo is required for pathway stimulation.
After 24 hours the cells were changed to 0.5% serum-containing medium, and Hh protein or compounds were added; 24 hours later the cells were either monitored for luciferase activity using the Luc-lite assay kit (Packard Instrument Company, Meriden, USA) or harvested for RNA isolation using an RNA isolation kit (Qiagen; Valencia, USA).