Example sentences for: hhbss

How can you use “hhbss” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • MDCK cells grown on MatTek plates were quiesced overnight in serum-free medium, washed with HHBSS containing 1 mM probenecid and incubated with 5 μM Fura2-AM (Calbiochem) in HHBSS, 1 mM probenecid, and 1% DMSO for 45 min at 37°C.

  • U0126 or vehicle was added to each well and the cells were then incubated for 15 min at 37°C in 5% CO 2 . Cells were washed to remove unincorporated [ 3H]-AA and then incubated in HHBSS supplemented with 0.05% BSA with either U0126 or vehicle.

  • EYFP-cPLA 2 /ECFP-cPLA 2 S505A- or pECFP-cPLA 2 S727A-transfected MDCK cells grown on MatTek plates were quiesced overnight in serum-free medium, washed with and incubated in Hank's balanced salt solution (HBSS) additionally buffered with 25 mM HEPES pH 7.4 (HHBSS).

  • Cells were then washed with HHBSS containing 1 mM probenecid and imaged after a 30 min incubation for de-esterification of the Fura2-AM.


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