Words similar to hh-
Example sentences for: hh-
How can you use “hh-” in a sentence? Here are some example sentences to help you improve your vocabulary:
With the exception of the inactive derivative, these compounds all compete out the binding of [ 3H]-Hh-Ag 1.5 (0.
To explore this possibility we tested whether a tritiated form of the agonist analog Hh-Ag 1.5 could form a complex with Smo, when Smo is transiently overexpressed in 293T cells.
It has recently been shown that primary neonatal cerebellar granule neuron (CGN) precursors proliferate in response to Hh stimulation [ 2 ] . To determine whether the Hh agonist could elicit this response, we monitored [ 3H]-thymidine incorporation of cultured rat CGN precursors treated with Hh protein, Hh-Ag 1.1, Hh-Ag 1.2, or vehicle (DMSO).
5 μg total protein) were incubated in polypropylene tubes with 2 nM [ 3H]-Hh-Ag 1.5 in the presence or absence of 2 μM competitor in binding buffer (50 mM Tris pH 7.5, 5 mM MgCl, 1 mM EDTA, 0.1 % bovine serum albumin) plus EDTA-free protease inhibitor cocktail (Roche) in a final volume of 250 μl for 1-26 hours at 37°C.
To perform theses studies, Smo-overexpressing 293T cells were incubated for 2 hours at 37°C with 5 nM [ 3H]-Hh-Ag 1.5 in the presence of either KAAD-cyclopamine at 5 μM (Figure 6a, column 6), the related but inactive plant compound tomatadine at 5 μM (Figure 6a; Antag control 1, column 7), Cur61414 at 5 μM (Figure 6a, column 8), or a related but inactive Cur61414 derivative (Figure 6a; Antag control 2, column 9) at 5 μM.