Words similar to hexamers
Example sentences for: hexamers
How can you use “hexamers” in a sentence? Here are some example sentences to help you improve your vocabulary:
First strand cDNA was synthesized from 1 μg of total RNA in a 100 μl reaction volume using random hexamers as primer and Multiscribe reverse transcriptase (TaqMan Reverse Transcription Reagents Kit, PE Biosystems).
In the seminal paper [ 5 ] , Unger et al . analyzed hexamers and identified about 100 structural building blocks, in which, each amino acid has a position dependent probability of occurrence.
For first strand synthesis, RNA (200 ng) in 9 μl water was denatured at 65°C for 4 min, rapidly cooled on ice, and then resuspended in a 20 μl final volume containing 50 mM Tris-HCl pH 8.3, 75 mM KCl, 3 mM MgCl 2 , 10 mM DTT, 1 mM of each dNTP, 20 mM random hexamers and 200 U Superscript II RNase H -RT (GIBCO BRL, Gaithersburg, MD, USA).
Incubation of the RNA and random hexamers was performed at 95°C for 5 minutes and then held at 4°C.
A total of 9.68 μl of the RT mix was added to the RNA samples (with the random hexamers) and then subjected to RT cycle conditions (42°C for 45 minutes, 95°C for 5 minutes and a 4°C hold).