Words similar to hexamers
Example sentences for: hexamers
How can you use “hexamers” in a sentence? Here are some example sentences to help you improve your vocabulary:
Samples of total RNA (2 μg) from rat livers were reverse transcribed according to the SuperScript' First-Strand Synthesis System (Invitrogen, Carlsbad, CA) using random hexamers with a final volume of 20 μl.
0 mRNA Isolation Kit (Invitrogen Corp) and cDNA was subsequently generated using random hexamers as primers and S UPERS CRIPTII™ RNase H -Reverse Transcriptase (Invitrogen Corp).
Total RNA was purified from dissected nephron segments using Tri Reagent (Molecular Research Center) and reverse-transcribed to single-stranded cDNA using Moloney murine leukemia virus reverse transcriptase and 2.5 μM of random hexamers according to the manufacturer's protocol (GeneAmp RNA PCR kit, Perkin Elmer Cetus, Norwalk, CT).
Word counting is less prone to these sources of error, and uses information intrinsic to biases in codon usage by counting codon pairs as hexamers in a sliding window, whereas codons are read in non-overlapping, tiled windows.
First-strand cDNA synthesis was primed with 4 μg of random nonamers (New England Biolabs) or 6 μg random hexamers (Life Technologies) by heating at 70°C for 10 minutes, snap-cooling in dry ice/ethanol for 30 sec, and incubating at room temperature for an additional 5-10 min.