Words similar to hexamers
Example sentences for: hexamers
How can you use “hexamers” in a sentence? Here are some example sentences to help you improve your vocabulary:
First strand cDNA was synthesized from 1 μg of total RNA in a 100 μl reaction volume using random hexamers as primer and Multiscribe reverse transcriptase (TaqMan Reverse Transcription Reagents Kit, PE Biosystems).
RNA for cDNA production was performed using Promega's (Madison, WI) random hexamers, RNAsin, RT buffer, and RT enzyme.
Samples of total RNA (2 μg) from rat livers were reverse transcribed according to the SuperScript' First-Strand Synthesis System (Invitrogen, Carlsbad, CA) using random hexamers with a final volume of 20 μl.
For first strand synthesis, RNA (200 ng) in 9 μl water was denatured at 65°C for 4 min, rapidly cooled on ice, and then resuspended in a 20 μl final volume containing 50 mM Tris-HCl pH 8.3, 75 mM KCl, 3 mM MgCl 2 , 10 mM DTT, 1 mM of each dNTP, 20 mM random hexamers and 200 U Superscript II RNase H -RT (GIBCO BRL, Gaithersburg, MD, USA).
In the seminal paper [ 5 ] , Unger et al . analyzed hexamers and identified about 100 structural building blocks, in which, each amino acid has a position dependent probability of occurrence.