Words similar to hexamers

Example sentences for: hexamers

How can you use “hexamers” in a sentence? Here are some example sentences to help you improve your vocabulary:

Total RNA was purified from dissected nephron segments using Tri Reagent (Molecular Research Center) and reverse-transcribed to single-stranded cDNA using Moloney murine leukemia virus reverse transcriptase and 2.5 μM of random hexamers according to the manufacturer's protocol (GeneAmp RNA PCR kit, Perkin Elmer Cetus, Norwalk, CT).
For first strand synthesis, RNA (200 ng) in 9 μl water was denatured at 65°C for 4 min, rapidly cooled on ice, and then resuspended in a 20 μl final volume containing 50 mM Tris-HCl pH 8.3, 75 mM KCl, 3 mM MgCl 2 , 10 mM DTT, 1 mM of each dNTP, 20 mM random hexamers and 200 U Superscript II RNase H -RT (GIBCO BRL, Gaithersburg, MD, USA).
This second round of cDNA synthesis was similar to the first round except that random hexamers were used in priming of first-strand synthesis, with T7-(dT) 24 oligomer priming the second-strand.
0 mRNA Isolation Kit (Invitrogen Corp) and cDNA was subsequently generated using random hexamers as primers and S UPERS CRIPTII™ RNase H -Reverse Transcriptase (Invitrogen Corp).
First-strand cDNA synthesis was primed with 4 μg of random nonamers (New England Biolabs) or 6 μg random hexamers (Life Technologies) by heating at 70°C for 10 minutes, snap-cooling in dry ice/ethanol for 30 sec, and incubating at room temperature for an additional 5-10 min.