Words similar to hexamers
Example sentences for: hexamers
How can you use “hexamers” in a sentence? Here are some example sentences to help you improve your vocabulary:
Random hexamers were used as primers for reverse transcription of 2 μg of rat RNA from the multiple tissues.
First-strand cDNA synthesis was primed with 4 μg of random nonamers (New England Biolabs) or 6 μg random hexamers (Life Technologies) by heating at 70°C for 10 minutes, snap-cooling in dry ice/ethanol for 30 sec, and incubating at room temperature for an additional 5-10 min.
Reverse transcription of total RNA was performed after Dnase I digestion (Roche Diagnostics, Indianapolis, IN) with the Superscript™ II Rnase H -Reverse Transcriptase (GibcoBRL Life Technologies) protocol for cDNA synthesis using 2 μg of total RNA and 1 μg of random hexamers.
For each sample to be analyzed by microarray, 4 μg amplified RNA were primed with 5 μg random hexamers and 5 μg oligo dT and reverse-transcribed, incorporating aa-dUTP, as described at http://www.microarrays.org.
First strand cDNA was synthesized from 1 μg of total RNA in a 100 μl reaction volume using random hexamers as primer and Multiscribe reverse transcriptase (TaqMan Reverse Transcription Reagents Kit, PE Biosystems).