Example sentences for: hexamers

How can you use “hexamers” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • First strand cDNA was synthesized from 1 μg of total RNA in a 100 μl reaction volume using random hexamers as primer and Multiscribe reverse transcriptase (TaqMan Reverse Transcription Reagents Kit, PE Biosystems).

  • RNA for cDNA production was performed using Promega's (Madison, WI) random hexamers, RNAsin, RT buffer, and RT enzyme.

  • Samples of total RNA (2 μg) from rat livers were reverse transcribed according to the SuperScript' First-Strand Synthesis System (Invitrogen, Carlsbad, CA) using random hexamers with a final volume of 20 μl.

  • For first strand synthesis, RNA (200 ng) in 9 μl water was denatured at 65°C for 4 min, rapidly cooled on ice, and then resuspended in a 20 μl final volume containing 50 mM Tris-HCl pH 8.3, 75 mM KCl, 3 mM MgCl 2 , 10 mM DTT, 1 mM of each dNTP, 20 mM random hexamers and 200 U Superscript II RNase H -RT (GIBCO BRL, Gaithersburg, MD, USA).

  • In the seminal paper [ 5 ] , Unger et al . analyzed hexamers and identified about 100 structural building blocks, in which, each amino acid has a position dependent probability of occurrence.


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