Words similar to hexamer
Example sentences for: hexamer
How can you use “hexamer” in a sentence? Here are some example sentences to help you improve your vocabulary:
The purified 5 μg aRNA was labeled with Cy3 or Cy5 by reverse transcription in a solution containing 10 μg of random hexamer, 4 μl first-strand reaction buffer, 2 μl 10 mM dithiothreitol (DTT; Gibco-BRL), 1 μl of 2 mM dATP, dGTP, dTTP and 1 mM dCTP, 1 μl SUPERase-in, (Amtion) and 200 u Superscript II reverse transcriptase (Gibco-BRL).
Also, filtering or trimming of low-complexity repeat regions, such as poly(A) or poly(T) tracts, is helpful because comparison results can be influenced by the abundance of a single hexamer.
The second round of amplification utilized 0.5 to 1 μg aRNA as starting material and resembled the first round, except for random hexamer pd(N)6 (Boehringer Mannheim, Igelheim, Germany) used in the reverse-transcription reaction in place of the template-switch primer.
Thus, we trimmed poly(A) and poly(T) sites to minimize the cases in which a test sequence resembles one training set more closely than the other, simply by virtue of having an abundance of the hexamer AAAAAA or TTTTTT.
In this protocol, we resampled each training set 40 times by random partitioning into training (for hexamer counts) and test calculation pools.
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