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Example sentences for: hel

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HEL cells were dislodged from the flask with a trypsin-EDTA solution, washed with DEPC-treated PBS, and then resuspended in 6 ml of ice-cold resuspension buffer [10 mM Tris-HCl (pH 7.5), 10 mM KC1, 5 mM MgCl 2 ]. Six ml of hot (70°C) lysis buffer [20 mM Tris-HCl (pH 7.4), 0.4 M NaCI, 40 mM EDTA, 1% β-mercaptoethanol, 1% SDS] and 1 ml of citrate-buffered phenol (pH 4.0) were added to each cell suspension.
Total IgE concentrations were slightly, but significantly, higher in the peptide-immunized groups (G7, PD, or HEL/OVA) compared to those in mice injected with IFA and saline alone (Figure 3).
Anti-peptide IgG1 and IgG2a antibodies also were detected in PD- (Figure 2B) and HEL/OVA- (Figure 2C) immunized mice, although the magnitude of the antibody responses to these peptides (especially the IgG1 response) were substantially less than those to the G7 peptides.
Three peptide pools consisted of: G7 (GAD 206-226, GAD 217-236, GAD 286-300), PD (GAD K458-470R, GAD 333-345), and hen egg lysozyme/ovalbumin (HEL/OVA; HEL 81-96, OVA 323-339).
This 5-day incubation was to allow sufficient time for P. carinii organisms to interact with the feeder layer (HEL cells) and for apoptosis of the short-lived rat-derived cells that were present in the inoculum.