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Example sentences for: hel

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Total IgE concentrations were slightly, but significantly, higher in the peptide-immunized groups (G7, PD, or HEL/OVA) compared to those in mice injected with IFA and saline alone (Figure 3).
HEL cells were dislodged from the flask with a trypsin-EDTA solution, washed with DEPC-treated PBS, and then resuspended in 6 ml of ice-cold resuspension buffer [10 mM Tris-HCl (pH 7.5), 10 mM KC1, 5 mM MgCl 2 ]. Six ml of hot (70°C) lysis buffer [20 mM Tris-HCl (pH 7.4), 0.4 M NaCI, 40 mM EDTA, 1% β-mercaptoethanol, 1% SDS] and 1 ml of citrate-buffered phenol (pH 4.0) were added to each cell suspension.
The RNAimage™ Kit (GenHunter Corporation, Nashville, TN) was used to detect differences between RNA samples of mock- (M) and P. carinii -infected (I) HEL cells.
However, the serum concentrations of total IgE were very similar in the mice that had been immunized with G7, PD or HEL/OVA peptides (Figure 3).
Anti-G7 IgG antibodies generally were not detectable in the other groups (PD- or HEL/OVA- immunized, or IFA alone) or in the non-injected (naïve) mice.