Words similar to hel
Example sentences for: hel
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immunization with peptides (10/12 in the G7 group; 3/4 for PD, 3/10 for HEL/OVA).
Total IgE concentrations were slightly, but significantly, higher in the peptide-immunized groups (G7, PD, or HEL/OVA) compared to those in mice injected with IFA and saline alone (Figure 3).
HEL cells were dislodged from the flask with a trypsin-EDTA solution, washed with DEPC-treated PBS, and then resuspended in 6 ml of ice-cold resuspension buffer [10 mM Tris-HCl (pH 7.5), 10 mM KC1, 5 mM MgCl 2 ]. Six ml of hot (70°C) lysis buffer [20 mM Tris-HCl (pH 7.4), 0.4 M NaCI, 40 mM EDTA, 1% β-mercaptoethanol, 1% SDS] and 1 ml of citrate-buffered phenol (pH 4.0) were added to each cell suspension.
Notably, in the mice immunized with G7, PD or HEL/OVA emulsified in IFA, anaphylactic responses were also provoked by the third i.p.
Anti-peptide IgG1 and IgG2a antibodies also were detected in PD- (Figure 2B) and HEL/OVA- (Figure 2C) immunized mice, although the magnitude of the antibody responses to these peptides (especially the IgG1 response) were substantially less than those to the G7 peptides.